Inhibition of Growth and Induction of Apoptosis in Fibrosarcoma Cell Lines by Echinophora platyloba DC : In Vitro Analysis
المؤلفون المشاركون
Azadmehr, Abbas
Bandehagh, Ali
Hajiaghaee, Reza
Shahneh, Fatemeh Zare
Baradaran, Behzad
Valiyari, Samira
Yaripour, Saeid
المصدر
Advances in Pharmacological Sciences
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-01-14
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Echinophora platyloba DC plant (Khousharizeh) is one of the indigenous medicinal plants which is used as a food seasoning and medicine in Iran.
The objective of this study was to examine the in vitro cytotoxic activity and the mechanism of cell death of crude methanolic extracts prepared from Echinophora platyloba DC, on mouse fibrosarcoma cell line (WEHI-164).
Cytotoxicity and viability of methanolic extract was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and dye exclusion assay.
Cell death ELISA was employed to quantify the nucleosome production result from nuclear DNA fragmentation during apoptosis and determine whether the mechanism involves induction of apoptosis or necrosis.
The cell death was identified as apoptosis using terminal deoxynucleotidyl transferase- (TdT-) mediated dUTP nick end labeling (TUNEL) assay.
Our results demonstrated that the extract decreased cell viability, suppressed cell proliferation, and induced cell death in a time- and dose-dependent manner in WEHI-164 cells (IC50 = 196.673 ± 12.4 μg/mL) when compared with a chemotherapeutic anticancer drug, Toxol.
Observation proved that apoptosis was the major mechanism of cell death.
So the Echinophora platyloba DC extract was found to time- and dose-dependently inhibit the proliferation of fibrosarcoma cell possibly via an apoptosis-dependent pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Shahneh, Fatemeh Zare& Valiyari, Samira& Azadmehr, Abbas& Hajiaghaee, Reza& Yaripour, Saeid& Bandehagh, Ali…[et al.]. 2013. Inhibition of Growth and Induction of Apoptosis in Fibrosarcoma Cell Lines by Echinophora platyloba DC : In Vitro Analysis. Advances in Pharmacological Sciences،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-477556
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Shahneh, Fatemeh Zare…[et al.]. Inhibition of Growth and Induction of Apoptosis in Fibrosarcoma Cell Lines by Echinophora platyloba DC : In Vitro Analysis. Advances in Pharmacological Sciences No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-477556
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Shahneh, Fatemeh Zare& Valiyari, Samira& Azadmehr, Abbas& Hajiaghaee, Reza& Yaripour, Saeid& Bandehagh, Ali…[et al.]. Inhibition of Growth and Induction of Apoptosis in Fibrosarcoma Cell Lines by Echinophora platyloba DC : In Vitro Analysis. Advances in Pharmacological Sciences. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-477556
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-477556
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر