Ototoxicity from Combined Cisplatin and Radiation Treatment : An In Vitro Study
المؤلفون المشاركون
Low, Wong-Kein
Tan, Michelle G. K.
Kong, Sylvia W. W.
المصدر
International Journal of Otolaryngology
العدد
المجلد 2010، العدد 2010 (31 ديسمبر/كانون الأول 2010)، ص ص. 1-29، 29ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2010-12-01
دولة النشر
مصر
عدد الصفحات
29
التخصصات الرئيسية
الملخص EN
Objective.
Combined cisplatin (CDDP) and radiotherapy is increasingly being used to treat advanced head and neck cancers.
As both CDDP and radiation can cause hearing loss, it is important to have a better understanding of the cellular and molecular ototoxic mechanisms involved in combined therapy.
Procedure.
The effects of CDDP, radiation, and combined CDDP-radiation on the OC-k3 cochlear cell line were studied using MTS assay, flow cytometry, Western blotting, and microarray analysis.
Results.
Compared to using CDDP or radiation alone, its combined use resulted in enhanced apoptotic cell death and apoptotic-related gene expression, including that of FAS.
Phosphorylation of p53 at Ser15 (a marker for p53 pathway activation in response to DNA damage) was observed after treatment with either CDDP or radiation.
However, posttreatment activation of p53 occurred earlier in radiation than in CDDP which corresponded to the timings of MDM2 and TP53INP1 expression.
Conclusion.
Enhanced apoptotic-related gene expressions leading to increased apoptotic cell deaths could explain the synergistic ototoxicity seen clinically in combined CDDP-radiation therapy.
CDDP and radiation led to differential temporal activation of p53 which suggests that their activation is the result of different upstream processes.
These have implications in future antiapoptotic treatments for ototoxicity.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Low, Wong-Kein& Kong, Sylvia W. W.& Tan, Michelle G. K.. 2010. Ototoxicity from Combined Cisplatin and Radiation Treatment : An In Vitro Study. International Journal of Otolaryngology،Vol. 2010, no. 2010, pp.1-29.
https://search.emarefa.net/detail/BIM-478483
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Low, Wong-Kein…[et al.]. Ototoxicity from Combined Cisplatin and Radiation Treatment : An In Vitro Study. International Journal of Otolaryngology No. 2010 (2010), pp.1-29.
https://search.emarefa.net/detail/BIM-478483
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Low, Wong-Kein& Kong, Sylvia W. W.& Tan, Michelle G. K.. Ototoxicity from Combined Cisplatin and Radiation Treatment : An In Vitro Study. International Journal of Otolaryngology. 2010. Vol. 2010, no. 2010, pp.1-29.
https://search.emarefa.net/detail/BIM-478483
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-478483
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر