Design and Statistical Analysis of Pooled Next Generation Sequencing for Rare Variants
المؤلفون المشاركون
Wen, Ruofeng
Zhang, Yuanhao
Wang, Tao
Ye, Kenny
Lin, Chang-Yun
المصدر
Journal of Probability and Statistics
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-19، 19ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-09-05
دولة النشر
مصر
عدد الصفحات
19
التخصصات الرئيسية
الملخص EN
Next generation sequencing (NGS) is a revolutionary technology for biomedical research.
One highly cost-efficient application of NGS is to detect disease association based on pooled DNA samples.
However, several key issues need to be addressed for pooled NGS.
One of them is the high sequencing error rate and its high variability across genomic positions and experiment runs, which, if not well considered in the experimental design and analysis, could lead to either inflated false positive rates or loss in statistical power.
Another important issue is how to test association of a group of rare variants.
To address the first issue, we proposed a new blocked pooling design in which multiple pools of DNA samples from cases and controls are sequenced together on same NGS functional units.
To address the second issue, we proposed a testing procedure that does not require individual genotypes but by taking advantage of multiple DNA pools.
Through a simulation study, we demonstrated that our approach provides a good control of the type I error rate, and yields satisfactory power compared to the test-based on individual genotypes.
Our results also provide guidelines for designing an efficient pooled.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Tao& Lin, Chang-Yun& Zhang, Yuanhao& Wen, Ruofeng& Ye, Kenny. 2012. Design and Statistical Analysis of Pooled Next Generation Sequencing for Rare Variants. Journal of Probability and Statistics،Vol. 2012, no. 2012, pp.1-19.
https://search.emarefa.net/detail/BIM-478566
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Tao…[et al.]. Design and Statistical Analysis of Pooled Next Generation Sequencing for Rare Variants. Journal of Probability and Statistics No. 2012 (2012), pp.1-19.
https://search.emarefa.net/detail/BIM-478566
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Tao& Lin, Chang-Yun& Zhang, Yuanhao& Wen, Ruofeng& Ye, Kenny. Design and Statistical Analysis of Pooled Next Generation Sequencing for Rare Variants. Journal of Probability and Statistics. 2012. Vol. 2012, no. 2012, pp.1-19.
https://search.emarefa.net/detail/BIM-478566
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-478566
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر