Factors Influencing the Abundance of the Side Population in a Human Myeloma Cell Line

المؤلفون المشاركون

Brown, Ross D.
Joshua, Douglas
Ng, Michelle C. H.
Sze, Daniel Man-yuen
Li, Jia
Smith, Adrian L.
Roufogalis, Basil D.
Mo, Sui-Lin
Chen, Yuling
Fan, Kei
Li, George Q.
Loh, Yen S.

المصدر

Bone Marrow Research

العدد

المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-8، 8ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2011-09-22

دولة النشر

مصر

عدد الصفحات

8

التخصصات الرئيسية

الأحياء

الملخص EN

Side population (SP) refers to a group of cells, which is capable to efflux Hoechst 33342, a DNA-binding dye.

SP cells exist both in normal and tumor tissues.

Although SP abundance has been used as an indicator for disease prognostic and drug screening in many research projects, few studies have systematically examined the factors influencing SP analysis.

In this study we aim to develop a more thorough understanding of the multiple factors involved in SP analysis including Hoechst 33342 staining and cell culture.

RPMI-8226, a high SP percentage (SP%) human myeloma cell line was employed here.

The results showed that SP% was subject to staining conditions including: viable cell proportion, dye concentration, staining cell density, incubation duration, staining volume, and mix interval.

In addition, SP% was highest in day one after passage, while dropped steadily over time.

This study shows that both staining conditions and culture duration can significantly affect SP%.

In this case, any conclusions based on SP% should be interpreted cautiously.

The relation between culture duration and SP% suggests that the incidence of SP cells may be related to cell proliferation and cell cycle phase.

Maintaining these technical variables consistently is essential in SP research.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Mo, Sui-Lin& Li, Jia& Loh, Yen S.& Brown, Ross D.& Smith, Adrian L.& Chen, Yuling…[et al.]. 2011. Factors Influencing the Abundance of the Side Population in a Human Myeloma Cell Line. Bone Marrow Research،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-478575

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Mo, Sui-Lin…[et al.]. Factors Influencing the Abundance of the Side Population in a Human Myeloma Cell Line. Bone Marrow Research No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-478575

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Mo, Sui-Lin& Li, Jia& Loh, Yen S.& Brown, Ross D.& Smith, Adrian L.& Chen, Yuling…[et al.]. Factors Influencing the Abundance of the Side Population in a Human Myeloma Cell Line. Bone Marrow Research. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-478575

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-478575