α-RgIB : A Novel Antagonist Peptide of Neuronal Acetylcholine Receptor Isolated from Conus regius Venom
المؤلفون المشاركون
Braga, Maria Cristina Vianna
Nery, Arthur Andrade
Pimenta, Daniel Carvalho
Konno, Katsuhiro
Sciani, Juliana Mozer
Ulrich, Henning
المصدر
International Journal of Peptides
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-02-27
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Conus venoms are rich sources of biologically active peptides that act specifically on ionic channels and metabotropic receptors present at the neuromuscular junction, efficiently paralyzing the prey.
Each species of Conus may have 50 to 200 uncharacterized bioactive peptides with pharmacological interest.
Conus regius is a vermivorous species that inhabits Northeastern Brazilian tropical waters.
In this work, we characterized one peptide with activity on neuronal acetylcholine receptor (nAChR).
Crude venom was purified by reverse-phase HPLC and selected fractions were screened and sequenced by mass spectrometry, MALDI-ToF, and ESI-Q-ToF, respectively.
A new peptide was identified, bearing two disulfide bridges.
The novel 2,701 Da peptide belongs to the cysteine framework I, corresponding to the cysteine pattern CC-C-C.
The biological activity of the purified peptide was tested by intracranial injection in mice, and it was observed that high concentrations induced hyperactivity in the animals, whereas lower doses caused breathing difficulty.
The activity of this peptide was assayed in patch-clamp experiments, on nAChR-rich cells, in whole-cell configuration.
The peptide blocked slow rise-time neuronal receptors, probably α3β4 and/or α3β4α5 subtype.
According to the nomenclature, the new peptide was designated as α-RgIB.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Braga, Maria Cristina Vianna& Nery, Arthur Andrade& Ulrich, Henning& Konno, Katsuhiro& Sciani, Juliana Mozer& Pimenta, Daniel Carvalho. 2013. α-RgIB : A Novel Antagonist Peptide of Neuronal Acetylcholine Receptor Isolated from Conus regius Venom. International Journal of Peptides،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-480160
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Braga, Maria Cristina Vianna…[et al.]. α-RgIB : A Novel Antagonist Peptide of Neuronal Acetylcholine Receptor Isolated from Conus regius Venom. International Journal of Peptides No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-480160
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Braga, Maria Cristina Vianna& Nery, Arthur Andrade& Ulrich, Henning& Konno, Katsuhiro& Sciani, Juliana Mozer& Pimenta, Daniel Carvalho. α-RgIB : A Novel Antagonist Peptide of Neuronal Acetylcholine Receptor Isolated from Conus regius Venom. International Journal of Peptides. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-480160
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-480160
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر