Okadaic Acid, a Bioactive Fatty Acid from Halichondria okadai, Stimulates Lipolysis in Rat Adipocytes : The Pivotal Role of Perilipin Translocation
المؤلفون المشاركون
Chang, Nen-Chung
Hsu, Cheng-Chen
Fong, Tsorng-Harn
Chen, Chien-Yuan
Tsui, Leo
Liu, Jung-Sheng
Lin, Aming Chor-Ming
Jayakumar, Thanasekaran
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-11-10
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Lipid metabolism in visceral fat cells is correlated with metabolic syndrome and cardiovascular diseases.
Okadaic-acid, a 38-carbon fatty acid isolated from the black sponge Halichondria okadai, can stimulate lipolysis by promoting the phosphorylation of several proteins in adipocytes.
However, the mechanism of okadaic acid-induced lipolysis and the effects of okadaic acid on lipid-droplet-associated proteins (perilipins and beta-actin) remain unclear.
We isolated adipocytes from rat epididymal fat pads and treated them with isoproterenol and/or okadaic acid to estimate lipolysis by measuring glycerol release.
Incubating adipocytes with okadaic acid stimulated time-dependent lipolysis.
Lipid-droplet-associated perilipins and beta-actin were analyzed by immunoblotting and immunofluorescence, and the association of perilipin A and B was found to be decreased in response to isoproterenol or okadaic acid treatment.
Moreover, okadaic-acid treatment could enhance isoproterenol-mediated lipolysis, whereas treatment of several inhibitors such as KT-5720 (PKA inhibitor), calphostin C (PKC inhibitor), or KT-5823 (PKG inhibitor) did not attenuate okadaic-acid-induced lipolysis.
By contrast, vanadyl acetylacetonate (tyrosine phosphatase inhibitor) blocked okadaic-acid-dependent lipolysis.
These results suggest that okadaic acid induces the phosphorylation and detachment of lipid-droplet-associated perilipin A and B from the lipid droplet surface and thereby leads to accelerated lipolysis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chang, Nen-Chung& Lin, Aming Chor-Ming& Hsu, Cheng-Chen& Liu, Jung-Sheng& Tsui, Leo& Chen, Chien-Yuan…[et al.]. 2013. Okadaic Acid, a Bioactive Fatty Acid from Halichondria okadai, Stimulates Lipolysis in Rat Adipocytes : The Pivotal Role of Perilipin Translocation. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-480315
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chang, Nen-Chung…[et al.]. Okadaic Acid, a Bioactive Fatty Acid from Halichondria okadai, Stimulates Lipolysis in Rat Adipocytes : The Pivotal Role of Perilipin Translocation. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-480315
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chang, Nen-Chung& Lin, Aming Chor-Ming& Hsu, Cheng-Chen& Liu, Jung-Sheng& Tsui, Leo& Chen, Chien-Yuan…[et al.]. Okadaic Acid, a Bioactive Fatty Acid from Halichondria okadai, Stimulates Lipolysis in Rat Adipocytes : The Pivotal Role of Perilipin Translocation. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-480315
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-480315
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر