Induction of Boosted Immune Response in Mice by Leptospiral Surface Proteins Expressed in Fusion with DnaK
المؤلفون المشاركون
Leite, Luciana C. C.
Rodriguez, Dunia
Nascimento, Ana L. T. O.
Atzingen, Marina V.
Siqueira, Gabriela Hase
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-07-06
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Leptospirosis is an important global disease of human and veterinary concern.
Caused by pathogenic Leptospira, the illness was recently classified as an emerging infectious disease.
Currently available veterinarian vaccines do not induce long-term protection against infection and do not provide cross-protective immunity.
Several studies have suggested the use of DnaK as an antigen in vaccine formulation, due to an exceptional degree of immunogenicity.
We focused on four surface proteins: rLIC10368 (Lsa21), rLIC10494, rLIC12690 (Lp95), and rLIC12730, previously shown to be involved in host-pathogen interactions.
Our goal was to evaluate the immunogenicity of the proteins genetically fused with DnaK in animal model.
The chosen genes were amplified by PCR methodology and cloned into pAE, an E.
coli vector.
The recombinant proteins were expressed alone or in fusion with DnaK at the N-terminus.
Our results demonstrate that leptospiral proteins fused with DnaK have elicited an enhanced immune response in mice when compared to the effect promoted by the individual proteins.
The boosted immune effect was demonstrated by the production of total IgG, lymphocyte proliferation, and significant amounts of IL-10 in supernatant of splenocyte cell cultures.
We believe that this approach could be employed in vaccines to enhance presentation of antigens of Leptospira to professional immune cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Atzingen, Marina V.& Rodriguez, Dunia& Siqueira, Gabriela Hase& Leite, Luciana C. C.& Nascimento, Ana L. T. O.. 2014. Induction of Boosted Immune Response in Mice by Leptospiral Surface Proteins Expressed in Fusion with DnaK. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-481119
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Atzingen, Marina V.…[et al.]. Induction of Boosted Immune Response in Mice by Leptospiral Surface Proteins Expressed in Fusion with DnaK. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-481119
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Atzingen, Marina V.& Rodriguez, Dunia& Siqueira, Gabriela Hase& Leite, Luciana C. C.& Nascimento, Ana L. T. O.. Induction of Boosted Immune Response in Mice by Leptospiral Surface Proteins Expressed in Fusion with DnaK. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-481119
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-481119
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر