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Development of a Recombinant Cell-Based Indirect Immunofluorescence Assay for the Determination of Autoantibodies against Soluble Liver Antigen in Autoimmune Hepatitis
المؤلفون المشاركون
Radzimski, Christiane
Schlumberger, Wolfgang
Blöcker, Inga Madeleine
Bogdanos, Dimitrios P.
Teegen, Bianca
Komorowski, Lars
Rentzsch, Kristin
Probst, Christian
Dähnrich, Cornelia
Stöcker, Winfried
المصدر
Clinical and Developmental Immunology
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-01-16
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Autoantibodies against soluble liver antigen (SLA) are specific markers for autoimmune hepatitis (AIH) type 1.
In contrast to the determination of other AIH-associated autoantibodies by indirect immunofluorescence assay (IFA), detection of anti-SLA relied up to now on ELISA or immunoblot based on bacterially expressed recombinant protein.
In order to develop a complementary IFA substrate, SLA isoform 1 was recombinantly produced in the human cell line HEK293 and controlled by a rabbit hyperimmune serum against SLA.
The recombinant cells were used in IFA (RC-IFA) to analyze sera from 20 AIH patients with anti-SLA positivity predetermined by ELISA together with 80 controls (20 anti-SLA negative AIH, 15 primary biliary cirrhosis, 15 HCV, and 30 healthy blood donors).
Using RC-IFA, anti-SLA was detected in all ELISA positive AIH sera but in none of the controls.
Furthermore, a cytosolic fraction of HEK293 containing SLA was able to neutralize the autoantibodies in all positive sera in a dose-dependent manner.
HEK293 cells expressing SLA are a valid substrate for the serodiagnosis of AIH relevant autoantibodies by IFA.
In concert with cryosections of primate liver, rat kidney, rat liver, rat stomach, and HEp-2 cells, they enable the parallel determination of all autoantibodies associated with autoimmune liver diseases.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Radzimski, Christiane& Probst, Christian& Teegen, Bianca& Rentzsch, Kristin& Blöcker, Inga Madeleine& Dähnrich, Cornelia…[et al.]. 2013. Development of a Recombinant Cell-Based Indirect Immunofluorescence Assay for the Determination of Autoantibodies against Soluble Liver Antigen in Autoimmune Hepatitis. Clinical and Developmental Immunology،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-481799
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Radzimski, Christiane…[et al.]. Development of a Recombinant Cell-Based Indirect Immunofluorescence Assay for the Determination of Autoantibodies against Soluble Liver Antigen in Autoimmune Hepatitis. Clinical and Developmental Immunology No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-481799
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Radzimski, Christiane& Probst, Christian& Teegen, Bianca& Rentzsch, Kristin& Blöcker, Inga Madeleine& Dähnrich, Cornelia…[et al.]. Development of a Recombinant Cell-Based Indirect Immunofluorescence Assay for the Determination of Autoantibodies against Soluble Liver Antigen in Autoimmune Hepatitis. Clinical and Developmental Immunology. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-481799
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-481799
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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