Interferon Alpha Characterization and Its Comparative Expression in PBM Cells of Capra hircus and Antelope cervicapra Cultured in the Presence of TLR9 Agonist
المؤلفون المشاركون
Swarup, Devendra
Doreswamy, Ramesh
Upreti, Suchitra
Das, Asit
Saini, Mohini
Singh, Vivek Kumar
Gupta, Praveen K.
المصدر
Molecular Biology International
العدد
المجلد 2010، العدد 2010 (31 ديسمبر/كانون الأول 2010)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2010-06-03
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
العلوم الطبيعية والحياتية (متداخلة التخصصات)
الأحياء
الملخص EN
TLR9 plays pivotal role in innate immune responses through upregulation of costimulatory molecules and induction of proinflammatory cytokines like type I interferons including interferon alpha (IFNA).
The present study characterized IFNA cDNA and predicted protein sequences in goat and black buck.
Response of the PBM cells to TLR9 agonist CpG ODN C and Phorbol Myristate Acetate (PMA) was evaluated by realtime PCR.
IFNA coding sequences were amplified from leukocyte cDNA and cloned in pGEMT-easy vector for nucleotide sequencing.
Sequence analysis revealed 570 bp, IFNA ORF encoding 189 amino acids in goat and black buck.
Black buck and goat IFNA has 92.1% to 94.7% and 93% to 95.6% similarity at nucleotide level, 86.3% to 89.5% and 70.9% to 91.6% identity at amino acid level with other ruminants, respectively.
Nonsynonymous substitutions exceeding synonymous substitutions indicated IFNA evolved through positive selection among ruminants.
In spite of lower total leukocyte count, the innate immune cells like monocytes and neutrophils were more in black buck compared to goat.
In addition, CpG ODN C-stimulated PBM cells revealed raised IFNA transcript in black buck than goat.
These findings indicate sturdy genetically governed immune system in wild antelope black buck compared to domestic ruminant goat.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Doreswamy, Ramesh& Saini, Mohini& Swarup, Devendra& Singh, Vivek Kumar& Upreti, Suchitra& Das, Asit…[et al.]. 2010. Interferon Alpha Characterization and Its Comparative Expression in PBM Cells of Capra hircus and Antelope cervicapra Cultured in the Presence of TLR9 Agonist. Molecular Biology International،Vol. 2010, no. 2010, pp.1-6.
https://search.emarefa.net/detail/BIM-481870
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Doreswamy, Ramesh…[et al.]. Interferon Alpha Characterization and Its Comparative Expression in PBM Cells of Capra hircus and Antelope cervicapra Cultured in the Presence of TLR9 Agonist. Molecular Biology International No. 2010 (2010), pp.1-6.
https://search.emarefa.net/detail/BIM-481870
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Doreswamy, Ramesh& Saini, Mohini& Swarup, Devendra& Singh, Vivek Kumar& Upreti, Suchitra& Das, Asit…[et al.]. Interferon Alpha Characterization and Its Comparative Expression in PBM Cells of Capra hircus and Antelope cervicapra Cultured in the Presence of TLR9 Agonist. Molecular Biology International. 2010. Vol. 2010, no. 2010, pp.1-6.
https://search.emarefa.net/detail/BIM-481870
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-481870
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر