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Assessment of Chromatin Maturity in Human Spermatozoa : Useful Aniline Blue Assay for Routine Diagnosis of Male Infertility
المؤلفون المشاركون
Ammar-Keskes, L.
Sellami, Afifa
Kebaili, Sahbi
Sellami, Hanen
Ben Zarrouk, Soumaya
Chakroun, Nozha
Rebai, Tarek
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-10-03
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
During spermatogenesis, sperm chromatin undergoes structural changes and results in a high condensation.
This nuclear compaction would be useful as a predictor of sperm fertilization capacity and pregnancy outcome.
We purpose to evaluate firstly the relationship among chromatin maturity assessed by aniline blue staining (AB) and the semen parameters in infertile men.
Secondly, we analyzed whether the sperm gradient density centrifugation is effective to select mature spermatozoa.
Fifty-one ejaculates were investigated by semen analysis and stained for chromatin condensation with AB to distinguish between unstained mature sperm and stained immature sperm.
AB was applied also on 12 ejaculates which proceeded by density gradient centrifugation to compare the rates of immature sperm before and after selection.
Neat semen were divided into two groups: G1 (n=31): immature sperm <20% and G2 (n=20): immature sperm ≥20%.
No significant differences were detected in sperm concentration, motility, and normal morphology between G1 and G2.
However, the rates of some morphology abnormalities were higher in G2: head abnormalities (P=0.01) and microcephalic sperm (P=0.02).
We founded significant correlation between sperm immaturity and acrosome abnormalities (r=0.292; P=0.03).
Sperm selection has significantly reduced the rates of immature sperm.
A better understanding of chromatin structure and its impact on the sperm potential is needed to explore male infertility.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sellami, Afifa& Chakroun, Nozha& Ben Zarrouk, Soumaya& Sellami, Hanen& Kebaili, Sahbi& Rebai, Tarek…[et al.]. 2013. Assessment of Chromatin Maturity in Human Spermatozoa : Useful Aniline Blue Assay for Routine Diagnosis of Male Infertility. Advances in Urology،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-482244
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sellami, Afifa…[et al.]. Assessment of Chromatin Maturity in Human Spermatozoa : Useful Aniline Blue Assay for Routine Diagnosis of Male Infertility. Advances in Urology No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-482244
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sellami, Afifa& Chakroun, Nozha& Ben Zarrouk, Soumaya& Sellami, Hanen& Kebaili, Sahbi& Rebai, Tarek…[et al.]. Assessment of Chromatin Maturity in Human Spermatozoa : Useful Aniline Blue Assay for Routine Diagnosis of Male Infertility. Advances in Urology. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-482244
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-482244
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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