Electroacupuncture Regulates ApoptosisProliferation of Intramuscular Interstitial Cells of Cajal and Restores Colonic Motility in Diabetic Constipation Rats
المؤلفون المشاركون
Xu, Juanjuan
Chen, Yan
Liu, Shi
Hou, Xiaohua
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-11-18
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Injury of interstitial cells of Cajal (ICC) is associated with gut dysmotility in diabetic rats.
We have shown an acceleration of the colonic contractility by electroacupuncture stimulation (EAS).
However, little is known about potential roles of EAS on colonic transit and ICC.
In this study, we evaluate the effect of EAS on colonic transit and investigate whether apoptosis/proliferation of ICC was involved in regulative effect of EAS on colonic transit.
Rats were randomly assigned to normal, diabetic, diabetic-plus-sham stimulation, diabetic-plus-low-frequency stimulation, and diabetic-plus-high-frequency stimulation groups.
Bead expulsion test was used for measuring the distal colonic transit.
The Kit (ICC marker) was detected by western blot.
Apoptotic ICC was detected by terminal dUTP nucleotide end labeling.
Proliferating ICC was identified by Kit/Ki67 double immunofluorescent staining on whole mount preparations.
Ultrastructure changes of ICC were studied using electron microscopy.
Results showed that high-frequency stimulation significantly promoted colonic transit.
Low- and high-frequency stimulation markedly rescued intramuscular ICC from apoptosis.
Abundant proliferating intramuscular ICC was found in low- and high-frequency stimulation groups.
Our results indicate that high-frequency EAS has stimulatory effect on the distal colonic transit, which may be mediated by downregulation of the apoptosis and upregulation of the proliferation of intramuscular ICC.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xu, Juanjuan& Chen, Yan& Liu, Shi& Hou, Xiaohua. 2013. Electroacupuncture Regulates ApoptosisProliferation of Intramuscular Interstitial Cells of Cajal and Restores Colonic Motility in Diabetic Constipation Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-482741
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xu, Juanjuan…[et al.]. Electroacupuncture Regulates ApoptosisProliferation of Intramuscular Interstitial Cells of Cajal and Restores Colonic Motility in Diabetic Constipation Rats. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-482741
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xu, Juanjuan& Chen, Yan& Liu, Shi& Hou, Xiaohua. Electroacupuncture Regulates ApoptosisProliferation of Intramuscular Interstitial Cells of Cajal and Restores Colonic Motility in Diabetic Constipation Rats. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-482741
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-482741
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر