Development of Eimeria nieschulzi (Coccidia, Apicomplexa) Gamonts and Oocysts in Primary Fetal Rat Cells
المؤلفون المشاركون
Hanig, Sacha
Kurth, Michael
Wiedmer, Stefanie
Chen, Hong
Entzeroth, Rolf
المصدر
Journal of Parasitology Research
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-06-19
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
The in vitro production of gametocytes and oocysts of the apicomplexan parasite genus Eimeria is still a challenge in coccidiosis research.
Until today, an in vitro development of gametocytes or oocysts had only been shown in some Eimeria species.
For several mammalian Eimeria species, partial developments could be achieved in different cell types, but a development up to gametocytes or oocysts is still lacking.
This study compares several permanent cell lines with primary fetal cells of the black rat (Rattus norvegicus) concerning the qualitative in vitro development of the rat parasite Eimeria nieschulzi.
With the help of transgenic parasites, the developmental progress was documented.
The selected Eimeria nieschulzi strain constitutively expresses the yellow fluorescent protein and a macrogamont specific upregulated red tandem dimer tomato.
In the majority of all investigated host cells the development stopped at the second merozoite stage.
In a mixed culture of cells derived from inner fetal organs the development of schizont generations I-IV, macrogamonts, and oocysts were observed in crypt-like organoid structures.
Microgamonts and microgametes could not be observed and oocysts did not sporulate under air supply.
By immunohistology, we could confirm that wild-type E.
nieschulzi stages can be found in the crypts of the small intestine.
The results of this study may be helpful for characterization of native host cells and for development of an in vitro cultivation system for Eimeria species.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Hong& Wiedmer, Stefanie& Hanig, Sacha& Entzeroth, Rolf& Kurth, Michael. 2013. Development of Eimeria nieschulzi (Coccidia, Apicomplexa) Gamonts and Oocysts in Primary Fetal Rat Cells. Journal of Parasitology Research،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-483340
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Hong…[et al.]. Development of Eimeria nieschulzi (Coccidia, Apicomplexa) Gamonts and Oocysts in Primary Fetal Rat Cells. Journal of Parasitology Research No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-483340
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Hong& Wiedmer, Stefanie& Hanig, Sacha& Entzeroth, Rolf& Kurth, Michael. Development of Eimeria nieschulzi (Coccidia, Apicomplexa) Gamonts and Oocysts in Primary Fetal Rat Cells. Journal of Parasitology Research. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-483340
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-483340
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر