Cost-Effective Production and Optimization of Alkaline Xylanase by Indigenous Bacillus mojavensis AG137 Fermented on Agricultural Waste
المؤلفون المشاركون
Akhavan Sepahy, Abbas
Akhavan Sepahy, Maryam
Ghazi, Shokoofeh
المصدر
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-08-29
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
A xylanase producer Bacillus mojavensis strain, called AG137, isolated from cotton farm (Kashan-Iran).
The optimal xylanase activity reached at 55∘C & pH 9.0.
Enzyme yield was studied using a medium with different agricultural wastes as inducers.
Xylanase production of about 249.308 IU/mL was achieved at pH 8 and 37∘C, within 48 h submerged fermentation in enzyme production medium supplemented with 2% (w/v) oat bran as an optimum carbon source.
A mixture of 1% (w/v) yeast extract and 1% (w/v) tryptone as optimum nitrogen sources, agitation speed 200 rpm, and inoculum size 2% (v/v) were the optimums for maximum production.
Accordingly, xylanase yield from 194.68 IU/mL under non-optimized fermentation condition enhanced to 302.466 IU/mL in optimized condition.
Screened xylanase is thermostable, presenting 70% stability at 60∘C during 30 min.
Further enzyme incubation in higher temperature caused a decrease in the residual enzyme activity, yet it retained 68%–50% of its activity after 1 hour from 45∘C to 55∘C.
Besides, it is stable in pH 9 and 10, maintaining over 70% of its activity for 2 h.
The enzyme also could preserve 71% and 63% of its initial activity after 3 hours of pre-incubation in the same alkaline condition.
Produced xylanase therefore was introduced as an alkaline-active and stable one, displaying suitable thermostability feature, confirmed by HPLC analysis.
Hence, all xylanase properties highlight its promising uses in industrial scale.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Akhavan Sepahy, Abbas& Ghazi, Shokoofeh& Akhavan Sepahy, Maryam. 2011. Cost-Effective Production and Optimization of Alkaline Xylanase by Indigenous Bacillus mojavensis AG137 Fermented on Agricultural Waste. Enzyme Research،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-483556
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Akhavan Sepahy, Abbas…[et al.]. Cost-Effective Production and Optimization of Alkaline Xylanase by Indigenous Bacillus mojavensis AG137 Fermented on Agricultural Waste. Enzyme Research No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-483556
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Akhavan Sepahy, Abbas& Ghazi, Shokoofeh& Akhavan Sepahy, Maryam. Cost-Effective Production and Optimization of Alkaline Xylanase by Indigenous Bacillus mojavensis AG137 Fermented on Agricultural Waste. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-483556
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-483556
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر