Identification of Human Tissue Kallikrein 6 as a Potential Marker of Laryngeal Cancer Based on the Relevant SecretoryReleasing Protein Database
المؤلفون المشاركون
Zhang, Zongmin
Tang, Pingzhang
Zhang, Ying
Xu, Bin
Cheng, Shujun
Gao, Yanning
Zhang, Zaixing
Han, Naijun
Li, Weihua
Xiao, Ting
Yang, Lei
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-02-11
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Objective.
This study was aimed to create a large-scale laryngeal cancer relevant secretory/releasing protein database and further discover candidate biomarkers.
Methods.
Primary tissue cultures were established using tumor tissues and matched normal mucosal tissues collected from four laryngeal cancer patients.
Serum-free conditioned medium (CM) samples were collected.
These samples were then sequentially processed by SDS-PAGE separation, trypsin digestion, and LC-MS/MS analysis.
The candidates in the database were validated by ELISA using plasma samples from laryngeal cancer patients, benign patients, and healthy individuals.
Results.
Combining MS data from the tumor tissues and normal tissues, 982 proteins were identified in total; extracellular proteins and cell surface proteins accounted for 15.0% and 4.3%, respectively.
According to stringent criteria, 49 proteins were selected as candidates worthy of further validation.
Of these, human tissue kallikrein 6 (KLK6) was verified.
The level of KLK6 was significantly increased in the plasma samples from the cancer cohort compared to the benign and healthy cohorts and moreover showed a slight decrease in the postoperative plasma samples in comparison to the preoperative plasma samples.
Conclusions.
This laryngeal cancer-derived protein database provides a promising repository of candidate blood biomarkers for laryngeal cancer.
The diagnostic potential of KLK6 deserves further investigation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Ying& Zhang, Zaixing& Yang, Lei& Xu, Bin& Li, Weihua& Tang, Pingzhang…[et al.]. 2014. Identification of Human Tissue Kallikrein 6 as a Potential Marker of Laryngeal Cancer Based on the Relevant SecretoryReleasing Protein Database. Disease Markers،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-483597
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Ying…[et al.]. Identification of Human Tissue Kallikrein 6 as a Potential Marker of Laryngeal Cancer Based on the Relevant SecretoryReleasing Protein Database. Disease Markers No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-483597
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Ying& Zhang, Zaixing& Yang, Lei& Xu, Bin& Li, Weihua& Tang, Pingzhang…[et al.]. Identification of Human Tissue Kallikrein 6 as a Potential Marker of Laryngeal Cancer Based on the Relevant SecretoryReleasing Protein Database. Disease Markers. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-483597
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-483597
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر