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Direct Quantitative Detection and Identification of Lactococcal Bacteriophages from Milk and Whey by Real-Time PCR : Application for the Detection of Lactococcal Bacteriophages in Goat's Raw Milk Whey in France
المؤلفون المشاركون
Vera, Annabelle
Ly-Chatain, Mai Huong
Demarigny, Yann
Rigobello, Véronique
Durand, Loïc
المصدر
International Journal of Microbiology
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-10-13
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
العلوم الطبيعية والحياتية (متداخلة التخصصات)
الطب البشري
الملخص EN
The presence of Lactococcus bacteriophages in milk can partly or completely inhibit milk fermentation.
To prevent the problems associated with the bacteriophages, the real-time PCR was developed in this study for direct detection from whey and milk of three main groups of Lactococcus bacteriophages, c2, 936, and P335.
The optimization of DNA extraction protocol from complex matrices such as whey and milk was optimized allowed the amplification of PCR without any matrix and nontarget contaminant interference.
The real-time PCR program was specific and with the detection limit of 102 PFU/mL.
The curve slopes were −3.49, −3.69, and −3.45 with the amplification efficiency estimated at 94%, 94%, and 98% and the correlation coefficient (R2) of 0.999, 0.999, and 0.998 for c2, 936 and P335 group, respectively.
This method was then used to detect the bacteriophages in whey and goat's raw milk coming from three farms located in the Rhône-Alpes region (France).
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ly-Chatain, Mai Huong& Durand, Loïc& Rigobello, Véronique& Vera, Annabelle& Demarigny, Yann. 2011. Direct Quantitative Detection and Identification of Lactococcal Bacteriophages from Milk and Whey by Real-Time PCR : Application for the Detection of Lactococcal Bacteriophages in Goat's Raw Milk Whey in France. International Journal of Microbiology،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-483623
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ly-Chatain, Mai Huong…[et al.]. Direct Quantitative Detection and Identification of Lactococcal Bacteriophages from Milk and Whey by Real-Time PCR : Application for the Detection of Lactococcal Bacteriophages in Goat's Raw Milk Whey in France. International Journal of Microbiology No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-483623
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ly-Chatain, Mai Huong& Durand, Loïc& Rigobello, Véronique& Vera, Annabelle& Demarigny, Yann. Direct Quantitative Detection and Identification of Lactococcal Bacteriophages from Milk and Whey by Real-Time PCR : Application for the Detection of Lactococcal Bacteriophages in Goat's Raw Milk Whey in France. International Journal of Microbiology. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-483623
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-483623
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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