Isolation and Biochemical Characterization of a New Thrombin-Like Serine Protease from Bothrops pirajai Snake Venom
المؤلفون المشاركون
Albericio, Fernando
Caballol, Maria Antonia O.
da Silva, Saulo Luís
Kayano, Anderson M.
Honório, Kathia M.
Acosta, Gerardo
Stábeli, Rodrigo G.
de Oliveira, Eliandre
Moreira-Dill, Leandro S.
Calderon, Leonardo A.
Fuly, André Lopes
Soares, Andreimar Martins
Maltarollo, Vinícius G.
Zaqueo, Kayena D.
Simões-Silva, Rodrigo
Fernandes, Carla F. C.
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-02-26
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
This paper presents a novel serine protease (SP) isolated from Bothrops pirajai, a venomous snake found solely in Brazil that belongs to the Viperidae family.
The identified SP, named BpirSP-39, was isolated by three chromatographic steps (size exclusion, bioaffinity, and reverse phase chromatographies).
The molecular mass of BpirSP-39 was estimated by SDS-PAGE and confirmed by mass spectrometry (39,408.32 Da).
The protein was able to form fibrin networks, which was not observed in the presence of serine protease inhibitors, such as phenylmethylsulfonyl fluoride (PMSF).
Furthermore, BpirSP-39 presented considerable thermal stability and was apparently able to activate factor XIII of the blood coagulation cascade, unlike most serine proteases.
BpirSP-39 was capable of hydrolyzing different chromogenic substrates tested (S-2222, S-2302, and S-2238) while Cu2+ significantly diminished BspirSP-39 activity on the three tested substrates.
The enzyme promoted platelet aggregation and also exhibited fibrinogenolytic, fibrinolytic, gelatinolytic, and amidolytic activities.
The multiple alignment showed high sequence similarity to other thrombin-like enzymes from snake venoms.
These results allow us to conclude that a new SP was isolated from Bothrops pirajai snake venom.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zaqueo, Kayena D.& Kayano, Anderson M.& Simões-Silva, Rodrigo& Moreira-Dill, Leandro S.& Fernandes, Carla F. C.& Fuly, André Lopes…[et al.]. 2014. Isolation and Biochemical Characterization of a New Thrombin-Like Serine Protease from Bothrops pirajai Snake Venom. BioMed Research International،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-483695
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zaqueo, Kayena D.…[et al.]. Isolation and Biochemical Characterization of a New Thrombin-Like Serine Protease from Bothrops pirajai Snake Venom. BioMed Research International No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-483695
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zaqueo, Kayena D.& Kayano, Anderson M.& Simões-Silva, Rodrigo& Moreira-Dill, Leandro S.& Fernandes, Carla F. C.& Fuly, André Lopes…[et al.]. Isolation and Biochemical Characterization of a New Thrombin-Like Serine Protease from Bothrops pirajai Snake Venom. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-483695
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-483695
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر