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Tetrodotoxin Detection by a Surface Plasmon Resonance Sensor in Pufferfish Matrices and Urine
المؤلفون المشاركون
Taylor, Allen D.
Vaisocherová, Hana
Deeds, Jonathan
Jiang, Shaoyi
DeGrasse, Stacey
المصدر
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-10-09
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Tetrodotoxin (TTX) poisoning is most commonly associated with consumption of pufferfish.
TTX is a low molecular weight (~319 Da) neurotoxin that selectively blocks voltage-sensitive Na+-gated ion channels.
The standard method accepted worldwide for monitoring TTX toxicity in food matrices is the mouse bioassay.
Ethical concerns from live animal testing, low sample throughput, and analytical inaccuracies have led to the need for an alternative method.
We have previously established that surface plasmon resonance (SPR) sensors can quantify TTX in aqueous buffer samples by an antibody-based inhibition assay.
In this paper, we report the extension of the assay for the detection of TTX in both clinical- and food-relevant matrices.
The assay was optimized for application to three relevant complex matrices: pufferfish liver extract, pufferfish muscle extract, and human urine.
Matrix effects are discussed and calibration curves are presented.
Naturally contaminated pufferfish liver and muscle extracts were analyzed by the SPR method, and the data is compared to liquid-chromatography electrospray-ionization multiple reactions monitoring mass spectrometry (LC/ESI/MRM/MS) data.
Ten samples, including three from a poisoning incident, two control monkfish samples, and five toxic pufferfish samples, were analyzed using this method, and the data is compared to LC/ESI/MRM/MS analysis of the samples.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Taylor, Allen D.& Vaisocherová, Hana& Deeds, Jonathan& DeGrasse, Stacey& Jiang, Shaoyi. 2011. Tetrodotoxin Detection by a Surface Plasmon Resonance Sensor in Pufferfish Matrices and Urine. Journal of Sensors،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-484127
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Taylor, Allen D.…[et al.]. Tetrodotoxin Detection by a Surface Plasmon Resonance Sensor in Pufferfish Matrices and Urine. Journal of Sensors No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-484127
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Taylor, Allen D.& Vaisocherová, Hana& Deeds, Jonathan& DeGrasse, Stacey& Jiang, Shaoyi. Tetrodotoxin Detection by a Surface Plasmon Resonance Sensor in Pufferfish Matrices and Urine. Journal of Sensors. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-484127
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-484127
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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