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Cultivable Anaerobic Microbiota of Infected Root Canals
المؤلفون المشاركون
Takahashi, Nobuhiro
Ishida, Naoko
Hashimoto, Kazuhiro
Sato, Emika
Shoji, Megumi
Takeuchi, Yasuhisa
Sato, Takuichi
Yamaki, Keiko
Shimauchi, Hidetoshi
Matsuyama, Junko
المصدر
International Journal of Dentistry
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-5، 5ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-04-03
دولة النشر
مصر
عدد الصفحات
5
التخصصات الرئيسية
الملخص EN
Objective.
Periapical periodontitis is an infectious and inflammatory disease of the periapical tissues caused by oral bacteria invading the root canal.
In the present study, profiling of the microbiota in infected root canals was performed using anaerobic culture and molecular biological techniques for bacterial identification.
Methods.
Informed consent was obtained from all subjects (age ranges, 34–71 years).
Nine infected root canals with periapical lesions from 7 subjects were included.
Samples from infected root canals were collected, followed by anaerobic culture on CDC blood agar plates.
After 7 days, colony forming units (CFU) were counted and isolated bacteria were identified by 16S rRNA gene sequencing.
Results.
The mean bacterial count (CFU) in root canals was (0.5±1.1)×106 (range 8.0×101–3.1×106), and anaerobic bacteria were predominant (89.8%).
The predominant isolates were Olsenella (25.4%), Mogibacterium (17.7%), Pseudoramibacter (17.7%), Propionibacterium (11.9%) and Parvimonas (5.9%).
Conclusion.
The combination of anaerobic culture and molecular biological techniques makes it possible to analyze rapidly the microbiota in infected root canals.
The overwhelming majority of the isolates from infected root canals were found to be anaerobic bacteria, suggesting that the environment in root canals is anaerobic and therefore support the growth of anaerobes.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sato, Takuichi& Yamaki, Keiko& Ishida, Naoko& Hashimoto, Kazuhiro& Takeuchi, Yasuhisa& Shoji, Megumi…[et al.]. 2012. Cultivable Anaerobic Microbiota of Infected Root Canals. International Journal of Dentistry،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-484872
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sato, Takuichi…[et al.]. Cultivable Anaerobic Microbiota of Infected Root Canals. International Journal of Dentistry No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-484872
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sato, Takuichi& Yamaki, Keiko& Ishida, Naoko& Hashimoto, Kazuhiro& Takeuchi, Yasuhisa& Shoji, Megumi…[et al.]. Cultivable Anaerobic Microbiota of Infected Root Canals. International Journal of Dentistry. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-484872
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-484872
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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