Semi-Automated Library Preparation for High-Throughput DNA Sequencing Platforms

المؤلفون المشاركون

Farias-Hesson, Eveline
Shen, Peidong
Erikson, Jonathan
Scharfe, Curt
Pourmand, Nader
Atkins, Alexander
Davis, Ronald W.

المصدر

Journal of Biomedicine and Biotechnology

العدد

المجلد 2010، العدد 2010 (31 ديسمبر/كانون الأول 2010)، ص ص. 1-8، 8ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2010-06-08

دولة النشر

مصر

عدد الصفحات

8

التخصصات الرئيسية

الطب البشري

الملخص EN

Next-generation sequencing platforms are powerful technologies, providing gigabases of genetic information in a single run.

An important prerequisite for high-throughput DNA sequencing is the development of robust and cost-effective preprocessing protocols for DNA sample library construction.

Here we report the development of a semi-automated sample preparation protocol to produce adaptor-ligated fragment libraries.

Using a liquid-handling robot in conjunction with Carboxy Terminated Magnetic Beads, we labeled each library sample using a unique 6 bp DNA barcode, which allowed multiplex sample processing and sequencing of 32 libraries in a single run using Applied Biosystems' SOLiD sequencer.

We applied our semi-automated pipeline to targeted medical resequencing of nuclear candidate genes in individuals affected by mitochondrial disorders.

This novel method is capable of preparing as much as 32 DNA libraries in 2.01 days (8-hour workday) for emulsion PCR/high throughput DNA sequencing, increasing sample preparation production by 8-fold.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Farias-Hesson, Eveline& Erikson, Jonathan& Atkins, Alexander& Shen, Peidong& Davis, Ronald W.& Scharfe, Curt…[et al.]. 2010. Semi-Automated Library Preparation for High-Throughput DNA Sequencing Platforms. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-485497

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Farias-Hesson, Eveline…[et al.]. Semi-Automated Library Preparation for High-Throughput DNA Sequencing Platforms. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-8.
https://search.emarefa.net/detail/BIM-485497

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Farias-Hesson, Eveline& Erikson, Jonathan& Atkins, Alexander& Shen, Peidong& Davis, Ronald W.& Scharfe, Curt…[et al.]. Semi-Automated Library Preparation for High-Throughput DNA Sequencing Platforms. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-485497

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-485497