Pharmacological Characterization of Inositol 1,4,5-tris Phosphate Receptors in Human Platelet Membranes
المؤلفون المشاركون
Pandey, Ghanshyam N.
Dwivedi, Yogesh
المصدر
Cardiovascular Psychiatry and Neurology
العدد
المجلد 2009، العدد 2009 (31 ديسمبر/كانون الأول 2009)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2009-10-12
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
The phosphatidylinositol (PI) hydrolysis signaling system has been shown to be altered in platelets of depressed and schizophrenic subjects.
Inositol (1,4,5) trisphosphate (Ins(1,4,5)P3), an integral component of the PI signaling system, mobilizes Ca2+ by activating Ins(1,4,5)P3 receptors.
To eventually investigate the role of Ins(1,4,5)P3 receptors in depression and other mental disorders, we characterized [H3]Ins(1,4,5)P3 binding sites in crude platelet membranes prepared from small amounts of blood obtained from healthy human control subjects.
We found a single, saturable binding site for [H3]Ins(1,4,5)P3 to crude platelet membranes, which is time dependent and modulated by pH, inositol phosphates, and heparin.
Since cyclic adenosine monophosphate (cAMP) and Ca2+ have been shown to be important modulators in Ins(1,4,5)P3 receptors, in the present study we also determined the effects of various concentrations of CaCI2 and forskolin on Ins(1,4,5)P3 binding to platelet membranes.
CaCI2 modulated [3H]Ins(1,4,5)P3 binding sites in a biphasic manner: at lower concentrations it inhibited [3H]Ins(1,4,5)P3 binding, whereas at higher concentrations, it stimulated [3H]Ins(1,4,5)P3 binding.
On the other hand, forskolin inhibited [3H]Ins(1,4,5)P3 binding.
Our results thus suggest that the pharmacological characteristics of [3H]Ins(1,4,5)P3 binding to crude platelet membranes are similar to that of Ins(1,4,5)P3 receptors; and that both Ca2+ and cAMP modulate [3H]Ins(1,4,5)P3 binding in crude platelet membranes.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Dwivedi, Yogesh& Pandey, Ghanshyam N.. 2009. Pharmacological Characterization of Inositol 1,4,5-tris Phosphate Receptors in Human Platelet Membranes. Cardiovascular Psychiatry and Neurology،Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-485594
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Dwivedi, Yogesh& Pandey, Ghanshyam N.. Pharmacological Characterization of Inositol 1,4,5-tris Phosphate Receptors in Human Platelet Membranes. Cardiovascular Psychiatry and Neurology No. 2009 (2009), pp.1-8.
https://search.emarefa.net/detail/BIM-485594
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Dwivedi, Yogesh& Pandey, Ghanshyam N.. Pharmacological Characterization of Inositol 1,4,5-tris Phosphate Receptors in Human Platelet Membranes. Cardiovascular Psychiatry and Neurology. 2009. Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-485594
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-485594
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر