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Characterization of Uptake and Internalization of Exosomes by Bladder Cancer Cells
المؤلفون المشاركون
Van Huis, Adam F.
Simms, Patricia E.
Gupta, Gopal N.
Kuo, Paul C.
Franzen, Carrie A.
Foreman, Kimberly E.
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-01-19
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Bladder tumors represent a special therapeutic challenge as they have a high recurrence rate requiring repeated interventions and may progress to invasive or metastatic disease.
Exosomes carry proteins implicated in bladder cancer progression and have been implicated in bladder cancer cell survival.
Here, we characterized exosome uptake and internalization by human bladder cancer cells using Amnis ImageStreamX, an image cytometer.
Exosomes were isolated by ultracentrifugation from bladder cancer culture conditioned supernatant, labeled with PKH-26, and analyzed on the ImageStreamX with an internal standard added to determine concentration.
Exosomes were cocultured with bladder cancer cells and analyzed for internalization.
Using the IDEAS software, we determined exosome uptake based on the number of PKH-26+ spots and overall PKH-26 fluorescence intensity.
Using unlabeled beads of a known concentration and size, we were able to determine concentrations of exosomes isolated from bladder cancer cells.
We measured exosome uptake by recipient bladder cancer cells, and we demonstrated that uptake is dose and time dependent.
Finally, we found that uptake is active and specific, which can be partially blocked by heparin treatment.
The characterization of cellular uptake and internalization by bladder cancer cells may shed light on the role of exosomes on bladder cancer recurrence and progression.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Franzen, Carrie A.& Simms, Patricia E.& Van Huis, Adam F.& Foreman, Kimberly E.& Kuo, Paul C.& Gupta, Gopal N.. 2014. Characterization of Uptake and Internalization of Exosomes by Bladder Cancer Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-485721
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Franzen, Carrie A.…[et al.]. Characterization of Uptake and Internalization of Exosomes by Bladder Cancer Cells. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-485721
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Franzen, Carrie A.& Simms, Patricia E.& Van Huis, Adam F.& Foreman, Kimberly E.& Kuo, Paul C.& Gupta, Gopal N.. Characterization of Uptake and Internalization of Exosomes by Bladder Cancer Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-485721
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-485721
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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