Induction of Mitochondrial Changes Associated with Oxidative Stress on Very Long Chain Fatty Acids (C22 : 0, C24:0, or C26:0)-Treated Human Neuronal Cells (SK-NB-E)
المؤلفون المشاركون
Haddad, Madudah
Nury, Thomas
Zarrouk, Amira
Cherkaoui-Malki, Mustapha
Vejux, Anne
Hammami, Mohamed
Lizard, Gérard
El Hajj, Hammam I.
Riedinger, Jean-Marc
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-08-05
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
العلوم الطبيعية والحياتية (متداخلة التخصصات)
الأحياء
الملخص EN
In Alzheimer's disease, lipid alterations point towards peroxisomal dysfunctions.
Indeed, a cortical accumulation of saturated very long chain fatty acids (VLCFAs: C22:0, C24:0, C26:0), substrates for peroxisomal β-oxidation, has been found in Alzheimer patients.
This study was realized to investigate the effects of VLCFAs at the mitochondrial level since mitochondrial dysfunctions play crucial roles in neurodegeneration.
On human neuronal SK-NB-E cells treated with C22:0, C24:0, or C26:0 (0.1–20 μM; 48 h), an inhibition of cell growth and mitochondrial dysfunctions were observed by cell counting with trypan blue, MTT assay, and measurement of mitochondrial transmembrane potential (Δψm) with DiOC6(3).
A stimulation of oxidative stress was observed with DHE and MitoSOX used to quantify superoxide anion production on whole cells and at the mitochondrial level, respectively.
With C24:0 and C26:0, by Western blotting, lower levels of mitochondrial complexes III and IV were detected.
After staining with MitoTracker and by transmission electron microscopy used to study mitochondrial topography, mass and morphology, major changes were detected in VLCFAs treated-cells: modification of the cytoplasmic distribution of mitochondria, presence of large mitochondria, enhancement of the mitochondrial mass.
Thus, VLCFAs can be potential risk factors contributing to neurodegeneration by inducing neuronal damages via mitochondrial dysfunctions.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zarrouk, Amira& Vejux, Anne& Nury, Thomas& El Hajj, Hammam I.& Haddad, Madudah& Cherkaoui-Malki, Mustapha…[et al.]. 2012. Induction of Mitochondrial Changes Associated with Oxidative Stress on Very Long Chain Fatty Acids (C22 : 0, C24:0, or C26:0)-Treated Human Neuronal Cells (SK-NB-E). Oxidative Medicine and Cellular Longevity،Vol. 2012, no. 2012, pp.1-15.
https://search.emarefa.net/detail/BIM-485949
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zarrouk, Amira…[et al.]. Induction of Mitochondrial Changes Associated with Oxidative Stress on Very Long Chain Fatty Acids (C22 : 0, C24:0, or C26:0)-Treated Human Neuronal Cells (SK-NB-E). Oxidative Medicine and Cellular Longevity No. 2012 (2012), pp.1-15.
https://search.emarefa.net/detail/BIM-485949
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zarrouk, Amira& Vejux, Anne& Nury, Thomas& El Hajj, Hammam I.& Haddad, Madudah& Cherkaoui-Malki, Mustapha…[et al.]. Induction of Mitochondrial Changes Associated with Oxidative Stress on Very Long Chain Fatty Acids (C22 : 0, C24:0, or C26:0)-Treated Human Neuronal Cells (SK-NB-E). Oxidative Medicine and Cellular Longevity. 2012. Vol. 2012, no. 2012, pp.1-15.
https://search.emarefa.net/detail/BIM-485949
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-485949
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر