A New Generation Microarray for the Simultaneous Detection and Identification of Yersinia pestis and Bacillus anthracis in Food

الملخص EN

The use of microarrays as a multiple analytic system has generated increased interest and provided a powerful analytical tool for the simultaneous detection of pathogens in a single experiment.

A wide array of applications for this technology has been reported.

A low density oligonucleotide microarray was generated from the genetic sequences of Y.

pestis and B.

anthracis and used to fabricate a microarray chip.

The new generation chip, consisting of 2,240 spots in 4 quadrants with the capability of stripping/rehybridization, was designated as “Y-PESTIS/B-ANTHRACIS 4x2K Array.” The chip was tested for specificity using DNA from a panel of bacteria that may be potentially present in food.

In all, 37 unique Y.

pestis-specific and 83 B.

anthracis-specific probes were identified.

The microarray assay distinguished Y.

pestis and B.

anthracis from the other bacterial species tested and correctly identified the Y.

pestis-specific oligonucleotide probes using DNA extracted from experimentally inoculated milk samples.

Using a whole genome amplification method, the assay was able to detect as low as 1 ng genomic DNA as the start sample.

The results suggest that oligonucleotide microarray can specifically detect and identify Y.

pestis and B.

anthracis and may be a potentially useful diagnostic tool for detecting and confirming the organisms in food during a bioterrorism event.