Association of Transferable Quinolone Resistance Determinant qnrB19 with Extended-Spectrum β-Lactamases in Salmonella Give and Salmonella Heidelberg in Venezuela

الملخص EN

Four nontyphoidal Salmonella strains with resistance to extended-spectrum cephalosporins and nonclassical quinolone resistance phenotype were studied.

Two S.

Give were isolated from pediatric patients with acute gastroenteritis, and two S.

Heidelberg were recovered from raw chicken meat.

Phenotypic characterization included antimicrobial susceptibility testing and detection of extended-spectrum β-lactamases (ESBLs) by the double-disc synergy method.

The detection of quinolone resistance-determining regions (QRDR) of gyrA, gyrB, and gyrC genes, blaESBLs genes, and plasmid-mediated quinolone resistance (PMQR) determinants was carried out by molecular methods.

Plasmid analysis included Southern blot and restriction patterns.

Transferability of resistance genes was examined by transformation.

blaTEM-1 + blaSHV-12 genes were detected in S.

Give SG9611 and blaTEM-1 + blaCTX-M-2 in the other three strains: S.

Give SG9811, S.

Heidelberg SH7511, and SH7911.

Regardless of origin and serovars, the qnrB19 gene was detected in the 4 strains studied.

All determinants of resistance were localized in plasmids and successfully transferred by transformation.

This study highlights the circulation of qnrB19 associated with blaTEM-1, blaSHV-12, and blaCTX-M-2 in S.

Give and S.

Heidelberg in Venezuela.

The recognition of factors associated with increasing resistance and the study of the molecular mechanisms involved can lead to a more focused use of antimicrobial agents.