R-Loop Formation In Trans at an AGGAG Repeat

الملخص EN

Formation of RNA-DNA hybrid, or R-loop, was studied in vitro by transcribing an AGGAG repeat with T7 RNA polymerase.

When ribonuclease T1 was present, R-loop formation in cis was diminished, indicating that the transcript was separated from the template and reassociated with it.

The transcript was found to form an R-loop in trans with DNA comprising the AGGAG repeat, when the DNA was supercoiled.

Results of chemical modification indicated that the duplex opened at the AGGAG repeat under negative supercoiling.