Use of Synthetic Single-Stranded Oligonucleotides as Artificial Test Soiling for Validation of Surgical Instrument Cleaning Processes
المؤلفون المشاركون
Wilhelm, Nadja
Avci-Adali, Meltem
Schlensak, Christian
Perle, Nadja
Simmoteit, Robert
Wendel, Hans-Peter
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-02-03
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Surgical instruments are often strongly contaminated with patients’ blood and tissues, possibly containing pathogens.
The reuse of contaminated instruments without adequate cleaning and sterilization can cause postoperative inflammation and the transmission of infectious diseases from one patient to another.
Thus, based on the stringent sterility requirements, the development of highly efficient, validated cleaning processes is necessary.
Here, we use for the first time synthetic single-stranded DNA (ssDNA_ODN), which does not appear in nature, as a test soiling to evaluate the cleaning efficiency of routine washing processes.
Stainless steel test objects were coated with a certain amount of ssDNA_ODN.
After cleaning, the amount of residual ssDNA_ODN on the test objects was determined using quantitative real-time PCR.
The established method is highly specific and sensitive, with a detection limit of 20 fg, and enables the determination of the cleaning efficiency of medical cleaning processes under different conditions to obtain optimal settings for the effective cleaning and sterilization of instruments.
The use of this highly sensitive method for the validation of cleaning processes can prevent, to a significant extent, the insufficient cleaning of surgical instruments and thus the transmission of pathogens to patients.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wilhelm, Nadja& Perle, Nadja& Simmoteit, Robert& Schlensak, Christian& Wendel, Hans-Peter& Avci-Adali, Meltem. 2014. Use of Synthetic Single-Stranded Oligonucleotides as Artificial Test Soiling for Validation of Surgical Instrument Cleaning Processes. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-486724
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wilhelm, Nadja…[et al.]. Use of Synthetic Single-Stranded Oligonucleotides as Artificial Test Soiling for Validation of Surgical Instrument Cleaning Processes. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-486724
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wilhelm, Nadja& Perle, Nadja& Simmoteit, Robert& Schlensak, Christian& Wendel, Hans-Peter& Avci-Adali, Meltem. Use of Synthetic Single-Stranded Oligonucleotides as Artificial Test Soiling for Validation of Surgical Instrument Cleaning Processes. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-486724
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-486724
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر