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Coexpression of PPE 34.9 Antigen of Mycobacterium avium subsp. Paratuberculosis with Murine Interferon Gamma in HeLa Cell Line and Study of Their Immunogenicity in Murine Model
المؤلفون المشاركون
المصدر
Biotechnology Research International
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-02-10
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
تكنولوجيا المعلومات وعلم الحاسوب
الملخص EN
Mycobacterium avium subsp.
paratuberculosis (Map) is the causative agent of johne's disease whose immunopathology mainly depends on cell mediated immuneresponse.
Genome sequencing revealed various PPE (Proline-Proline-Glutamic acid) protein family of Map which are immunologically importance candidate genes In present study we have developed a bicistrionic construct pIR PPE/IFN containing a 34.9 kDa PPE protein (PPE 34.9) of Map along with a cytokine gene encoding murine gamma Interferon gene (IFNγ) and a monocistrionic construct pIR PPE using a mammalian vector system pIRES 6.1.
The construct were transfected in HeLa cell line and expression were studied by Western blot as well as Immunefluroscent assay using recombinant sera.
Further we have compared the immunereactivity of these two constructs in murine model by means of DTH study, LTT, NO assay and ELISA.
DTH response was higher in pIR PPE/IFN than pIR PPE group of mice, similar finding also observed in case of LTT and NO production assay .
ELISA titer of the pIR PPE/IFN was less than that with PPE only.
These preliminary finding can revealed a CMI response of this PPE protein of Map and IFNγ having synergistic effect on this PPE protein to elicit a T cell based immunity in mice.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Deb, Rajib& Goswami, P. P.. 2011. Coexpression of PPE 34.9 Antigen of Mycobacterium avium subsp. Paratuberculosis with Murine Interferon Gamma in HeLa Cell Line and Study of Their Immunogenicity in Murine Model. Biotechnology Research International،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-486778
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Deb, Rajib& Goswami, P. P.. Coexpression of PPE 34.9 Antigen of Mycobacterium avium subsp. Paratuberculosis with Murine Interferon Gamma in HeLa Cell Line and Study of Their Immunogenicity in Murine Model. Biotechnology Research International No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-486778
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Deb, Rajib& Goswami, P. P.. Coexpression of PPE 34.9 Antigen of Mycobacterium avium subsp. Paratuberculosis with Murine Interferon Gamma in HeLa Cell Line and Study of Their Immunogenicity in Murine Model. Biotechnology Research International. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-486778
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-486778
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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