Impact of Novel Resistance Profiles in HIV-1 Reverse Transcriptase on Phenotypic Resistance to NVP
المؤلفون المشاركون
Li, Lin
Li, Hanping
Li, Jingyun
Liu, Siyang
Bao, Zuoyi
Liu, Yongjian
Zhuang, Daomin
Jiao, Liyan
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-03-21
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Objective.
To clarify the impact of H221Y mutation on drug resistance to NVP.
Methods.
646 bp HIV-1 pol gene fragments (from 592 to 1237 nucleotide) with different NNRTIs mutation profiles from AIDS patients receiving antiretroviral therapy containing NVP regimens were introduced into pNL4-3 backbone plasmid.
H221Y and (or) Y181C mutations were reverted to wild type amino acids by site-directed mutagenesis, then strains containing various mutation patterns were packaged.
Phenotypic drug resistance was analyzed on TZM-bl cells.
Results.
12 strains containing different drug-resistant mutation profiles were constructed, including the K101Q series (K101Q/Y181C/H221Y, K101Q/Y181C, K101Q/H221Y, and K101Q), the V179D series (V179D/Y181C/H221Y, V179D/Y181C, V179D/H221Y, and V179D), and the K103N series (K103N/Y181C/H221Y, K103N/Y181C, K103N/H221Y, K103N).
For strains containing the mutation profiles (K101Q/Y181C, K101Q, V179D/Y181C, V179D, K103N/Y181C, and K103N), the presence of H221Y reduced NVP susceptibility by 2.1±0.5 to 3.6±0.5 fold.
To the mutation profiles K101Q/H221Y, K101Q, V179D/H221Y, V179D, K103N/H221Y, and K103N, the presence of Y181C reduced NVP susceptibility by 41.9±8.4 to 1297.0±289.1 fold.
For the strains containing K101Q, V179D, and K103N, the presence of Y181C/H221Y combination decreased NVP susceptibility by 100.6±32.5 to 3444.6±834.5 fold.
Conclusion.
On the bases of various NNRTIs mutation profiles, Y181C remarkably improved the IC50 to NVP, although H221Ymutation alone just increases 2.1 ∼ 3.6-fold resistance to NVP, the mutation could improve 100.6 ∼ 3444.6-fold resistance to NVP when it copresent with Y181C, the phenotypic drug resistance fold was improved extremely.
For strains containing the mutation profiles (K101Q/Y181C, K101Q, V179D/Y181C, V179D, K103N/Y181C, and K103N), the presence of H221Y reduced NVP susceptibility by 2.1±0.5 to 3.6±0.5 fold.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jiao, Liyan& Li, Hanping& Li, Lin& Zhuang, Daomin& Liu, Yongjian& Bao, Zuoyi…[et al.]. 2012. Impact of Novel Resistance Profiles in HIV-1 Reverse Transcriptase on Phenotypic Resistance to NVP. AIDS Research and Treatment،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-487119
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jiao, Liyan…[et al.]. Impact of Novel Resistance Profiles in HIV-1 Reverse Transcriptase on Phenotypic Resistance to NVP. AIDS Research and Treatment No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-487119
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jiao, Liyan& Li, Hanping& Li, Lin& Zhuang, Daomin& Liu, Yongjian& Bao, Zuoyi…[et al.]. Impact of Novel Resistance Profiles in HIV-1 Reverse Transcriptase on Phenotypic Resistance to NVP. AIDS Research and Treatment. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-487119
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-487119
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر