Pharmacological Characterization of the Mechanisms Involved in Delayed Calcium Deregulation in SH-SY5Y Cells Challenged with Methadone
المؤلفون المشاركون
Cuenca-Lopez, Maria D.
Kmita, Hanna
Jordán Bueso, Joaquín
Villalobos, Carlos
Galindo, Maria F.
Perez-Alvarez, Sergio
Solesio, Maria E.
Melero-Fernández de Mera, Raquel M.
المصدر
International Journal of Cell Biology
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-06-17
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Previously, we have shown that SH-SY5Y cells exposed to high concentrations of methadone died due to a necrotic-like cell death mechanism related to delayed calcium deregulation (DCD).
In this study, we show that, in terms of their Ca2+ responses to 0.5 mM methadone, SH-SY5Y cells can be pooled into four different groups.
In a broad pharmacological survey, the relevance of different Ca2+-related mechanisms on methadone-induced DCD was investigated including extracellular calcium, L-type Ca2+ channels, μ-opioid receptor, mitochondrial inner membrane potential, mitochondrial ATP synthesis, mitochondrial Ca2+/2Na+-exchanger, reactive oxygen species, and mitochondrial permeability transition.
Only those compounds targeting mitochondria such as oligomycin, FCCP, CGP 37157, and cyclosporine A were able to amend methadone-induced Ca2+ dyshomeostasis suggesting that methadone induces DCD by modulating the ability of mitochondria to handle Ca2+.
Consistently, mitochondria became dramatically shorter and rounder in the presence of methadone.
Furthermore, analysis of oxygen uptake by isolated rat liver mitochondria suggested that methadone affected mitochondrial Ca2+ uptake in a respiratory substrate-dependent way.
We conclude that methadone causes failure of intracellular Ca2+ homeostasis, and this effect is associated with morphological and functional changes of mitochondria.
Likely, this mechanism contributes to degenerative side effects associated with methadone treatment.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Perez-Alvarez, Sergio& Solesio, Maria E.& Cuenca-Lopez, Maria D.& Melero-Fernández de Mera, Raquel M.& Villalobos, Carlos& Kmita, Hanna…[et al.]. 2012. Pharmacological Characterization of the Mechanisms Involved in Delayed Calcium Deregulation in SH-SY5Y Cells Challenged with Methadone. International Journal of Cell Biology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-487599
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Perez-Alvarez, Sergio…[et al.]. Pharmacological Characterization of the Mechanisms Involved in Delayed Calcium Deregulation in SH-SY5Y Cells Challenged with Methadone. International Journal of Cell Biology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-487599
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Perez-Alvarez, Sergio& Solesio, Maria E.& Cuenca-Lopez, Maria D.& Melero-Fernández de Mera, Raquel M.& Villalobos, Carlos& Kmita, Hanna…[et al.]. Pharmacological Characterization of the Mechanisms Involved in Delayed Calcium Deregulation in SH-SY5Y Cells Challenged with Methadone. International Journal of Cell Biology. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-487599
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-487599
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر