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Gene Delivery Potential of Biofunctional Carbonate Apatite Nanoparticles in Lungs
المؤلفون المشاركون
Alhaji, Suleiman Yusuf
Rosli, Rozita
Hassan, Fatma
Chowdhury, Ezharul Houque
Abdullah, Syahril
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-07-21
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Existing nonviral gene delivery systems to lungs are inefficient and associated with dose limiting toxicity in mammalian cells.
Therefore, carbonate apatite (CO3Ap) nanoparticles were examined as an alternative strategy for effective gene delivery to the lungs.
This study aimed to (1) assess the gene delivery efficiency of CO3Ap in vitro and in mouse lungs, (2) evaluate the cytotoxicity effect of CO3Ap/pDNA in vitro, and (3) characterize the CO3Ap/pDNA complex formulations.
A significantly high level of reporter gene expression was detected from the lung cell line transfected with CO3Ap/pDNA complex prepared in both serum and serum-free medium.
Cytotoxicity analysis revealed that the percentage of the viable cells treated with CO3Ap to be almost similar to the untreated cells.
Characterization analyses showed that the CO3Ap/pDNA complexes are in a nanometer range with aggregated spherical structures and tended to be more negatively charged.
In the lung of mice, highest level of transgene expression was observed when CO3Ap (8 μL) was complexed with 40 μg of pDNA at day 1 after administration.
Although massive reduction of gene expression was seen beyond day 1 post administration, the level of expression remained significant throughout the study period.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Alhaji, Suleiman Yusuf& Chowdhury, Ezharul Houque& Rosli, Rozita& Hassan, Fatma& Abdullah, Syahril. 2014. Gene Delivery Potential of Biofunctional Carbonate Apatite Nanoparticles in Lungs. BioMed Research International،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-487888
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Alhaji, Suleiman Yusuf…[et al.]. Gene Delivery Potential of Biofunctional Carbonate Apatite Nanoparticles in Lungs. BioMed Research International No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-487888
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Alhaji, Suleiman Yusuf& Chowdhury, Ezharul Houque& Rosli, Rozita& Hassan, Fatma& Abdullah, Syahril. Gene Delivery Potential of Biofunctional Carbonate Apatite Nanoparticles in Lungs. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-487888
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-487888
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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