In Vitro and In Vivo Effects of Suppressor of Cytokine Signalling 7 Knockdown in Breast Cancer : The Influence on Cellular Response to Hepatocyte Growth Factor
المؤلفون المشاركون
Jiang, Wen G.
Ye, Lin
Sasi, Walid
Sharma, Anup K.
Mokbel, Kefah
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-08-04
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Purpose.
Suppressor of cytokine signaling 7 (SOCS7) is a member of the SOCS family and is known to interact with phospholipase Cγ-1 (PLCγ-1), a key downstream mediator of the hepatocyte growth factor (HGF)/C-MET axis.
Here, we report our observations of the effect of knocking down SOCS7 gene on the behaviour of breast cancer cells both in vitro and in vivo and to elucidate whether this involves HGF/C-MET pathway using the PLCγ-1 blocker U73122.
Methods.
MCF7 and MDA-MB-231 breast cancer cells were transfected with anti-SOCS7 ribozymal transgene, to create sublines with SOCS7 knockdown.
The in vitro growth and migration of the cells were evaluated in basic conditions and with HGF and U73122 treatment using growth assays, scratch-wound, and electrical cell impedance sensing (ECIS) migration assays.
MCF7 and MDA-MB-231 in vivo tumour xenograft growth were also studied.
Results.
Basal in vitro growth and migration of both cellular lines and the in vivo MCF7 xenograft growth were significantly enhanced with SOCS7 knockdown.
In vitro HGF treatment has further influenced the growth and migration when SOCS7 gene was knocked-down in both cellular lines (P<0.05).
PLCγ-1 pharmacological inhibition of the HGF/C-MET cascade during their in vitro growth and migration seemed to only occur when SOCS7 gene was knocked down.
Conclusions.
We report a unique regulatory role for SOCS7 in controlling the malignant behaviour of breast cancer lines MCF7 and MDA-MB-231 in vitro and the MCF7 tumour xenografts in vivo.
We also report a regulatory role for SOCS7 during the in vitro HGF-induced growth and migration in these cells as HGF treatment and SOCS7 loss have synergistically enhanced these functions.
This SOCS7 knockdown-attributed effect could be due to a precise anti-PLCγ-1 role.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sasi, Walid& Ye, Lin& Jiang, Wen G.& Sharma, Anup K.& Mokbel, Kefah. 2014. In Vitro and In Vivo Effects of Suppressor of Cytokine Signalling 7 Knockdown in Breast Cancer : The Influence on Cellular Response to Hepatocyte Growth Factor. BioMed Research International،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-487988
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sasi, Walid…[et al.]. In Vitro and In Vivo Effects of Suppressor of Cytokine Signalling 7 Knockdown in Breast Cancer : The Influence on Cellular Response to Hepatocyte Growth Factor. BioMed Research International No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-487988
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sasi, Walid& Ye, Lin& Jiang, Wen G.& Sharma, Anup K.& Mokbel, Kefah. In Vitro and In Vivo Effects of Suppressor of Cytokine Signalling 7 Knockdown in Breast Cancer : The Influence on Cellular Response to Hepatocyte Growth Factor. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-487988
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-487988
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر