A Two-Tube Multiplex Reverse Transcription PCR Assay for Simultaneous Detection of Viral and Bacterial Pathogens of Infectious Diarrhea
المؤلفون المشاركون
Guan, Li
Wang, Ji
Ma, Xuejun
Niu, Peihua
Zhang, Chen
Xu, Ziqian
Zhang, Jingyun
Kan, Biao
Duan, Zhaojun
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-03-10
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Diarrhea caused by viral and bacterial infections is a major health problem in developing countries.
The purpose of this study is to develop a two-tube multiplex PCR assay using automatic electrophoresis for simultaneous detection of 13 diarrhea-causative viruses or bacteria, with an intended application in provincial Centers for Diseases Control and Prevention, China.
The assay was designed to detect rotavirus A, norovirus genogroups GI and GII, human astrovirus, enteric adenoviruses, and human bocavirus (tube 1), and Salmonella, Vibrio parahaemolyticus, diarrheagenic Escherichia coli, Campylobacter jejuni, Shigella, Yersinia, and Vibrio cholera (tube 2).
The analytical specificity was examined with positive controls for each pathogen.
The analytical sensitivity was evaluated by performing the assay on serial tenfold dilutions of in vitro transcribed RNA, recombinant plasmids, or bacterial culture.
A total of 122 stool samples were tested by this two-tube assay and the results were compared with those obtained from reference methods.
The two-tube assay achieved a sensitivity of 20–200 copies for a single virus and 102-103 CFU/mL for bacteria.
The clinical performance demonstrated that the two-tube assay had comparable sensitivity and specificity to those of reference methods.
In conclusion, the two-tube assay is a rapid, cost-effective, sensitive, specific, and high throughput method for the simultaneous detection of enteric bacteria and virus.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Ji& Xu, Ziqian& Niu, Peihua& Zhang, Chen& Zhang, Jingyun& Guan, Li…[et al.]. 2014. A Two-Tube Multiplex Reverse Transcription PCR Assay for Simultaneous Detection of Viral and Bacterial Pathogens of Infectious Diarrhea. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-488041
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Ji…[et al.]. A Two-Tube Multiplex Reverse Transcription PCR Assay for Simultaneous Detection of Viral and Bacterial Pathogens of Infectious Diarrhea. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-488041
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Ji& Xu, Ziqian& Niu, Peihua& Zhang, Chen& Zhang, Jingyun& Guan, Li…[et al.]. A Two-Tube Multiplex Reverse Transcription PCR Assay for Simultaneous Detection of Viral and Bacterial Pathogens of Infectious Diarrhea. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-488041
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-488041
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر