Evaluation of Distinct Freezing Methods and Cryoprotectants for Human Amniotic Fluid Stem Cells Cryopreservation
المؤلفون المشاركون
Zugaib, Marcelo
Cavaglieri, Rita de Cássia
Duarte, Sérgio Aloísio
Debes, Adriana de Aguiar
Bydlowski, Sérgio Paulo
Janz, Felipe de Lara
Moron, Antonio Fernandes
Romão, Carolina Martinez
المصدر
Journal of Biomedicine and Biotechnology
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-05-14
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Amniotic fluid (AF) was described as a potential source of mesenchymal stem cells (MSCs) for biomedicine purposes.
Therefore, evaluation of alternative cryoprotectants and freezing protocols capable to maintain the viability and stemness of these cells after cooling is still needed.
AF stem cells (AFSCs) were tested for different freezing methods and cryoprotectants.
Cell viability, gene expression, surface markers, and plasticity were evaluated after thawing.
AFSCs expressed undifferentiated genes Oct4 and Nanog; presented typical markers (CD29, CD44, CD90, and CD105) and were able to differentiate into mesenchymal lineages.
All tested cryoprotectants preserved the features of AFSCs however, variations in cell viability were observed.
In this concern, dimethyl sulfoxide (Me2SO) showed the best results.
The freezing protocols tested did not promote significant changes in the AFSCs viability.
Time programmed and nonprogrammed freezing methods could be used for successful AFSCs cryopreservation for 6 months.
Although tested cryoprotectants maintained undifferentiated gene expression, typical markers, and plasticity of AFSCs, only Me2SO and glycerol presented workable viability ratios.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Janz, Felipe de Lara& Debes, Adriana de Aguiar& Cavaglieri, Rita de Cássia& Duarte, Sérgio Aloísio& Romão, Carolina Martinez& Moron, Antonio Fernandes…[et al.]. 2012. Evaluation of Distinct Freezing Methods and Cryoprotectants for Human Amniotic Fluid Stem Cells Cryopreservation. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-488122
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Janz, Felipe de Lara…[et al.]. Evaluation of Distinct Freezing Methods and Cryoprotectants for Human Amniotic Fluid Stem Cells Cryopreservation. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-488122
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Janz, Felipe de Lara& Debes, Adriana de Aguiar& Cavaglieri, Rita de Cássia& Duarte, Sérgio Aloísio& Romão, Carolina Martinez& Moron, Antonio Fernandes…[et al.]. Evaluation of Distinct Freezing Methods and Cryoprotectants for Human Amniotic Fluid Stem Cells Cryopreservation. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-488122
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-488122
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر