The Role of EGFRPI3KAktcyclinD1 Signaling Pathway in Acquired Middle Ear Cholesteatoma
المؤلفون المشاركون
Ren, Jihao
Dai, Yinghuan
Xie, Shumin
Liu, Wei
Hu, Bing
Xie, Dinghua
Ren, Hongmiao
Yang, Xinming
Wu, Weijing
Yin, Tuanfang
Xiao, Zian
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-11-07
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Cholesteatoma is a benign keratinizing and hyper proliferative squamous epithelial lesion of the temporal bone.
Epidermal growth factor (EGF) is one of the most important cytokines which has been shown to play a critical role in cholesteatoma.
In this investigation, we studied the effects of EGF on the proliferation of keratinocytes and EGF-mediated signaling pathways underlying the pathogenesis of cholesteatoma.
We examined the expressions of phosphorylated EGF receptor (p-EGFR), phosphorylated Akt (p-Akt), cyclinD1, and proliferating cell nuclear antigen (PCNA) in 40 cholesteatoma samples and 20 samples of normal external auditory canal (EAC) epithelium by immunohistochemical method.
Furthermore, in vitro studies were performed to investigate EGF-induced downstream signaling pathways in primary external auditory canal keratinocytes (EACKs).
The expressions of p-EGFR, p-Akt, cyclinD1, and PCNA in cholesteatoma epithelium were significantly increased when compared with those of control subjects.
We also demonstrated that EGF led to the activation of the EGFR/PI3K/Akt/cyclinD1 signaling pathway, which played a critical role in EGF-induced cell proliferation and cell cycle progression of EACKs.
Both EGFR inhibitor AG1478 and PI3K inhibitor wortmannin inhibited the EGF-induced EGFR/PI3K/Akt/cyclinD1 signaling pathway concomitantly with inhibition of cell proliferation and cell cycle progression of EACKs.
Taken together, our data suggest that the EGFR/PI3K/Akt/cyclinD1 signaling pathway is active in cholesteatoma and may play a crucial role in cholesteatoma epithelial hyper-proliferation.
This study will facilitate the development of potential therapeutic targets for intratympanic drug therapy for cholesteatoma.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Wei& Ren, Hongmiao& Ren, Jihao& Yin, Tuanfang& Hu, Bing& Xie, Shumin…[et al.]. 2013. The Role of EGFRPI3KAktcyclinD1 Signaling Pathway in Acquired Middle Ear Cholesteatoma. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-488289
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Wei…[et al.]. The Role of EGFRPI3KAktcyclinD1 Signaling Pathway in Acquired Middle Ear Cholesteatoma. Mediators of Inflammation No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-488289
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Wei& Ren, Hongmiao& Ren, Jihao& Yin, Tuanfang& Hu, Bing& Xie, Shumin…[et al.]. The Role of EGFRPI3KAktcyclinD1 Signaling Pathway in Acquired Middle Ear Cholesteatoma. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-488289
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-488289
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر