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Upregulation of Transglutaminase and ε (γ-Glutamyl)-Lysine in the Fisher-Lewis Rat Model of Chronic Allograft Nephropathy
المؤلفون المشاركون
Shrestha, Badri Man
Johnson, Timothy
Butt, Imran
Da Silva, Michelle
Wagner, Bart
Sanchez-Lara, Armando
Raftery, Andrew
Haylor, John
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-07-21
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Background.
Tissue transglutaminase (TG2), a cross-linking enzyme, modulates deposition of extracellular matrix protein in renal fibrosis.
This study aimed to examine TG2 and its cross-link product ε(γ-glutamyl)-lysine in the Fisher-Lewis rat renal transplantation (RTx) model of chronic allograft nephropathy (CAN).
Materials and Methods.
Left renal grafts from male Fisher and Lewis were transplanted into Lewis rats, generating allografts and isografts, respectively.
Blood pressure, renal function, and proteinuria were monitored for up to 52 weeks.
At termination, CAN was assessed in the renal tissue by light and electron microscopy, TG2 and ε(γ-glutamyl)-lysine by immunofluorescence, and the urinary ε(γ-glutamyl)-lysine by high performance liquid chromatography.
Results.
Compared to the isograft, the allografts were hypertensive, proteinuric, and uraemic and developed CAN.
Extracellular TG2 (glomerulus: 64.55±17.61 versus 2.11±0.17, P<0.001; interstitium: 13.72±1.62 versus 3.19±0.44, P<0.001), ε(γ-glutamyl)-lysine (glomerulus: 21.74±2.71 versus 1.98±0.37, P<0.01; interstitium: 37.96±17.06 versus 0.42±0.11, P<0.05), TG2 enzyme activity (1.09±0.13 versus 0.41±0.03 nmol/h/mg protein, P<0.05), TG2 mRNA (20-fold rise), and urinary ε(γ-glutamyl)-lysine (534.2±198.4 nmol/24 h versus 57.2±4.1 nmol/24 h, P<0.05) levels were significantly elevated in the allografts and showed a positive linear correlation with tubulointerstitial fibrosis.
Conclusion.
CAN was associated with upregulation of renal TG2 pathway, which has a potential for pharmacological intervention.
The elevated urinary ε(γ-glutamyl)-lysine, measured for the first time in RTx, is a potential biomarker of CAN.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Shrestha, Badri Man& Butt, Imran& Da Silva, Michelle& Sanchez-Lara, Armando& Wagner, Bart& Raftery, Andrew…[et al.]. 2014. Upregulation of Transglutaminase and ε (γ-Glutamyl)-Lysine in the Fisher-Lewis Rat Model of Chronic Allograft Nephropathy. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-488331
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Shrestha, Badri Man…[et al.]. Upregulation of Transglutaminase and ε (γ-Glutamyl)-Lysine in the Fisher-Lewis Rat Model of Chronic Allograft Nephropathy. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-488331
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Shrestha, Badri Man& Butt, Imran& Da Silva, Michelle& Sanchez-Lara, Armando& Wagner, Bart& Raftery, Andrew…[et al.]. Upregulation of Transglutaminase and ε (γ-Glutamyl)-Lysine in the Fisher-Lewis Rat Model of Chronic Allograft Nephropathy. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-488331
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-488331
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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