Specific and Rapid Detection of Mycobacterium tuberculosis Complex in Clinical Samples by Polymerase Chain Reaction
المؤلفون المشاركون
Kashyap, Vijendra Kumar
Singh, Anamika
المصدر
Interdisciplinary Perspectives on Infectious Diseases
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-5، 5ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-10-09
دولة النشر
مصر
عدد الصفحات
5
التخصصات الرئيسية
الملخص EN
Background.
Tuberculosis, a global health problem and highly prevalent in India, has always been a serious problem with respect to definitive diagnosis.
Polymerase chain reaction (PCR) techniques are now widely used for early detection and species differentiation of mycobacteria, but mostly with their own limitations.
We aim to detect and differentiate Mycobacterium tuberculosis (Mtb) infections by choosing appropriate target sequences, ideally present in all mycobacterial species (MTB complex) and absent in others.
Methods.
Amplification of three target sequences from unrelated genes, namely, hsp 65 (165 bp), dnaJ (365 bp), and insertion element IS 6110 (541 bp) by PCR was carried out in clinical samples from suspected cases of tuberculosis/ mycobacterioses and healthy controls.
Results.
The sensitivity of this method ranged from 73.33% to 84.61%, and the specificity was 80%.
The PCR method was significantly better (P=0.03 and P=0.009) than both smear and culture methods.
Conclusion.
Our trimarker-based PCR method could specifically detect M.
tuberculosis and MTB complex infection from that of major pathogenic NTM and nonpathogenic mycobacteria.
This method, by well distinguishing between MTB complex and NTM, presented a fast and accurate method to detect and diagnose mycobacterial infections more efficiently and could thereby help in better patient management particularly considering the increase in mycobacterial infections due to emergence of NTM over the past decades.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Singh, Anamika& Kashyap, Vijendra Kumar. 2012. Specific and Rapid Detection of Mycobacterium tuberculosis Complex in Clinical Samples by Polymerase Chain Reaction. Interdisciplinary Perspectives on Infectious Diseases،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-488645
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Singh, Anamika& Kashyap, Vijendra Kumar. Specific and Rapid Detection of Mycobacterium tuberculosis Complex in Clinical Samples by Polymerase Chain Reaction. Interdisciplinary Perspectives on Infectious Diseases No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-488645
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Singh, Anamika& Kashyap, Vijendra Kumar. Specific and Rapid Detection of Mycobacterium tuberculosis Complex in Clinical Samples by Polymerase Chain Reaction. Interdisciplinary Perspectives on Infectious Diseases. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-488645
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-488645
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر