Plasmid Transfer of Plasminogen K1-5 Reduces Subcutaneous Hepatoma Growth by Affecting Inflammatory Factors
المؤلفون المشاركون
Strassburg, Christian P.
Koch, Lea A.
Raskopf, Esther
Schmitz, Volker
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-05-08
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
There is evidence that plasminogen K1-5 (PlgK1-5) directly affects tumour cells and inflammation.
Therefore, we analysed if PlgK1-5 has immediate effects on hepatoma cells and inflammatory factors in vitro and in vivo.
In vitro, effects of plasmid encoding PlgK1-5 (pK1-5) on Hepa129, Hepa1-6, and HuH7 cell viability, apoptosis, and proliferation as well as VEGF and TNF-alpha expression and STAT3-phosphorylation were investigated.
In vivo, tumour growth, proliferation, vessel density, and effects on vascular endothelial growth factor (VEGF) and tumour necrosis factor alpha (TNF-alpha) expression were examined following treatment with pK1-5.
In vivo, pK1-5 halved cell viability; cell death was increased by up to 15% compared to the corresponding controls.
Proliferation was not affected.
VEGF, TNF-alpha, and STAT3-phosphorylation were affected following treatment with pK1-5.
In vivo, ten days after treatment initiation, pK1-5 reduced subcutaneous tumour growth by 32% and mitosis by up to 77% compared to the controls.
Vessel density was reduced by 50%.
TNF-alpha levels in tumour and liver tissue were increased, whereas VEGF levels in tumours and livers were reduced after pK1-5 treatment.
Taken together, plasmid gene transfer of PlgK1-5 inhibits hepatoma (cell) growth not only by reducing vessel density but also by inducing apoptosis, inhibiting proliferation, and triggering inflammation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Koch, Lea A.& Schmitz, Volker& Strassburg, Christian P.& Raskopf, Esther. 2014. Plasmid Transfer of Plasminogen K1-5 Reduces Subcutaneous Hepatoma Growth by Affecting Inflammatory Factors. BioMed Research International،Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-488733
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Koch, Lea A.…[et al.]. Plasmid Transfer of Plasminogen K1-5 Reduces Subcutaneous Hepatoma Growth by Affecting Inflammatory Factors. BioMed Research International No. 2014 (2014), pp.1-14.
https://search.emarefa.net/detail/BIM-488733
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Koch, Lea A.& Schmitz, Volker& Strassburg, Christian P.& Raskopf, Esther. Plasmid Transfer of Plasminogen K1-5 Reduces Subcutaneous Hepatoma Growth by Affecting Inflammatory Factors. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-488733
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-488733
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر