The Ethanol Extract of Fructus trichosanthis Promotes Fetal Hemoglobin Production via p38 MAPK Activation and ERK Inactivation in K562 Cells
المؤلفون المشاركون
Tsang, Suk Ying
Leung, Ping Chung
Li, Hui
Fung, Kwok Pui
Kou, Chunhai
Fung, Ming Chui
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-08-11
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Pharmacological stimulation of fetal hemoglobin (HbF) expression may be a promising approach for the treatment of beta-thalassemia.
In this study, the effects of Fructus trichosanthis (FT) were investigated in human erythroleukemic K562 cells for their gamma-globin mRNA and HbF-induction activities.
The role of signaling pathways, including extracellular regulated protein kinase (ERK) and p38 mitogen-activated protein kinase (MAPK), was also investigated.
It was found that the ethanol extract of FT significantly increased gamma-globin mRNA and HbF levels, determined by real-time reverse transcription polymerase chain reaction and enzyme linked immunosorbent assay, respectively, in dose- and time-dependent manner.
Total Hb (THb) levels were also elevated in the concentrations without cytotoxicity (<80 μg mL−1).
Pre-treatment with p38 MAPK inhibitor SB203580 blocked the stimulatory effects of FT extract in total and HbF induction.
In contrast, no change in HbF was observed when treated with ERK inhibitor PD98059.
Furthermore, FT ethanol extract activated p38 MAPK and inhibited ERK signaling pathways in K562 cells, as revealed in western blotting analysis.
In addition, SB203580 significantly abolished p38 MAPK activation when the cells were treated with FT.
In summary, the ethanol extract of FT was found to be a potent inducer of HbF synthesis in K562 cells.
The present data delineated the role of ERK and p38 MAPK signaling as molecular targets for pharmacologic stimulation of HbF production upon FT treatment.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Hui& Kou, Chunhai& Tsang, Suk Ying& Leung, Ping Chung& Fung, Ming Chui& Fung, Kwok Pui. 2011. The Ethanol Extract of Fructus trichosanthis Promotes Fetal Hemoglobin Production via p38 MAPK Activation and ERK Inactivation in K562 Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-488763
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Hui…[et al.]. The Ethanol Extract of Fructus trichosanthis Promotes Fetal Hemoglobin Production via p38 MAPK Activation and ERK Inactivation in K562 Cells. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-488763
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Hui& Kou, Chunhai& Tsang, Suk Ying& Leung, Ping Chung& Fung, Ming Chui& Fung, Kwok Pui. The Ethanol Extract of Fructus trichosanthis Promotes Fetal Hemoglobin Production via p38 MAPK Activation and ERK Inactivation in K562 Cells. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-488763
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-488763
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر