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The ERK12 Inhibitor U0126 Attenuates Diabetes-Induced Upregulation of MMP-9 and Biomarkers of Inflammation in the Retina
المؤلفون المشاركون
Mairaj Siddiquei, Mohammad
Abu El-Asrar, Ahmed M.
Imtiaz Nawaz, Mohammad
Mohammad, Ghulam
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-04-10
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
This study was conducted to determine the expression of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in a time-dependent manner and the effect of extracellular-signal-regulated kinases-1/2 (ERK1/2) inhibition on the expressions of MMP-9, TIMP-1, and inflammatory biomarkers in the retinas of diabetic rats.
The expression of MMP-9 was quantified by zymography, and the mRNA level of MMP-9 and TIMP-1 was quantified by RT-PCR.
The expression of inducible nitric oxide synthase (iNOS), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α) was examined by Western blot analysis.
MMP-9 expression was significantly higher in diabetic rat retinas compared to controls at all time points.TIMP-1 expression was nonsignificantly upregulated at 1week of diabetes and was significantly downregulated at 4 and 12 weeks of diabetes.
Intravitreal administration of the ERK1/2 inhibitor U0126 prior to induction of diabetes decreased ERK1/2 activation, attenuated diabetes-induced upregulation of MMP-9, iNOS, IL-6, and TNF-α and upregulated TIMP-1 expression.
In MMP-9 knockout mice, diabetes had no effect on retinal iNOS expression and its level remained unchanged.
These data provide evidence that ERK1/2 signaling pathway is involved in MMP-9, iNOS, IL-6, and TNF-α induction in diabetic retinas and suggest that ERK1/2 can be a novel therapeutic target in diabetic retinopathy.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mohammad, Ghulam& Mairaj Siddiquei, Mohammad& Imtiaz Nawaz, Mohammad& Abu El-Asrar, Ahmed M.. 2013. The ERK12 Inhibitor U0126 Attenuates Diabetes-Induced Upregulation of MMP-9 and Biomarkers of Inflammation in the Retina. Journal of Diabetes Research،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-488914
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mohammad, Ghulam…[et al.]. The ERK12 Inhibitor U0126 Attenuates Diabetes-Induced Upregulation of MMP-9 and Biomarkers of Inflammation in the Retina. Journal of Diabetes Research No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-488914
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mohammad, Ghulam& Mairaj Siddiquei, Mohammad& Imtiaz Nawaz, Mohammad& Abu El-Asrar, Ahmed M.. The ERK12 Inhibitor U0126 Attenuates Diabetes-Induced Upregulation of MMP-9 and Biomarkers of Inflammation in the Retina. Journal of Diabetes Research. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-488914
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-488914
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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