Evaluation of the HB&L System for the Microbiological Screening of Storage Medium for Organ-Cultured Corneas
المؤلفون المشاركون
Frigo, Anna Chiara
Parekh, Mohit
Grandesso, S.
Camposampiero, Davide
Mazzucato, S.
Solinas, M.
Zanetti, E.
Gion, Massimo
Ponzin, Diego
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-5، 5ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-08-28
دولة النشر
مصر
عدد الصفحات
5
التخصصات الرئيسية
الملخص EN
Aims.
To compare HB&L and BACTEC systems for detecting the microorganisms contaminating the corneal storage liquid preserved at 31°C.
Methods.
Human donor corneas were stored at 4°C followed by preservation at 31°C.
Samples of the storage medium were inoculated in BACTEC Peds Plus/F (aerobic microorganisms), BACTEC Plus Anaerobic/F (anaerobic microorganisms), and HB&L bottles.
The tests were performed (a) after six days of storage, (b) end of storage, and (c) after 24 hours of preservation in deturgescent liquid sequentially.
10,655 storage and deturgescent media samples were subjected to microbiological control using BACTEC (6-day incubation) and HB&L (24-hour incubation) systems simultaneously.
BACTEC positive/negative refers to both/either aerobic and anaerobic positives/negatives, whereas HB&L can only detect the aerobic microbes, and therefore the positives/negatives depend on the presence/absence of aerobic microorganisms.
Results.
147 (1.38%) samples were identified positive with at least one of the two methods.
127 samples (134 identified microorganisms) were positive with both HB&L and BACTEC.
14 HB&L+/BACTEC− and 6 BACTEC+/HB&L− were identified.
Sensitivity (95.5%), specificity (99.8%), and positive (90.1%) and negative predictive values (99.9%) were high with HB&L considering a 3.5% annual contamination rate.
Conclusion.
HB&L is a rapid system for detecting microorganisms in corneal storage medium in addition to the existing methods.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Camposampiero, Davide& Grandesso, S.& Zanetti, E.& Mazzucato, S.& Solinas, M.& Parekh, Mohit…[et al.]. 2013. Evaluation of the HB&L System for the Microbiological Screening of Storage Medium for Organ-Cultured Corneas. Journal of Ophthalmology،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-489158
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Camposampiero, Davide…[et al.]. Evaluation of the HB&L System for the Microbiological Screening of Storage Medium for Organ-Cultured Corneas. Journal of Ophthalmology No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-489158
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Camposampiero, Davide& Grandesso, S.& Zanetti, E.& Mazzucato, S.& Solinas, M.& Parekh, Mohit…[et al.]. Evaluation of the HB&L System for the Microbiological Screening of Storage Medium for Organ-Cultured Corneas. Journal of Ophthalmology. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-489158
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-489158
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر