Combined Transfection of the Three Transcriptional Factors, PDX-1, NeuroD1, and MafA, Causes Differentiation of Bone Marrow Mesenchymal Stem Cells into Insulin-Producing Cells
المؤلفون المشاركون
Qing-Song, Guo
Xiang-Jun, Fan
Lei, Wang
Ming-Yan, Zhu
Sha-Jun, Zhu
Zhi-Wei, Wang
Yao, Wang
Yan, Huang
Yu-Hua, Lu
المصدر
Experimental Diabetes Research
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-06-19
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Aims.
The goal of cell transcription for treatment of diabetes is to generate surrogate β-cells from an appropriate cell line.
However, the induced replacement cells have showed less physiological function in producing insulin compared with normal β-cells.
Methods.
Here, we report a procedure for induction of insulin-producing cells (IPCs) from bone marrow murine mesenchymal stem cells (BM-mMSCs).
These BM-mMSCs have the potential to differentiate into insulin-producing cells when a combination of PDX-1 (pancreatic and duodenal homeobox-1), NeuroD1 (neurogenic differentiation-1), and MafA (V-maf musculoaponeurotic fibrosarcoma oncogene homolog A) genes are transfected into them and expressed in these cells.
Results.
Insulin biosynthesis and secretion were induced in mMSCs into which these three genes have been transfected and expressed.
The amount of induced insulin in the mMSCs which have been transfected with the three genes together is significantly higher than in those mMSCs that were only transfected with one or two of these three genes.
Transplantation of the transfected cells into mice with streptozotocin-induced diabetes results in insulin expression and the reversal of the glucose challenge.
Conclusions.
These findings suggest major implications for cell replacement strategies in generation of surrogate β-cells for the treatment of diabetes.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Qing-Song, Guo& Ming-Yan, Zhu& Lei, Wang& Xiang-Jun, Fan& Yu-Hua, Lu& Zhi-Wei, Wang…[et al.]. 2012. Combined Transfection of the Three Transcriptional Factors, PDX-1, NeuroD1, and MafA, Causes Differentiation of Bone Marrow Mesenchymal Stem Cells into Insulin-Producing Cells. Experimental Diabetes Research،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-489250
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Qing-Song, Guo…[et al.]. Combined Transfection of the Three Transcriptional Factors, PDX-1, NeuroD1, and MafA, Causes Differentiation of Bone Marrow Mesenchymal Stem Cells into Insulin-Producing Cells. Experimental Diabetes Research No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-489250
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Qing-Song, Guo& Ming-Yan, Zhu& Lei, Wang& Xiang-Jun, Fan& Yu-Hua, Lu& Zhi-Wei, Wang…[et al.]. Combined Transfection of the Three Transcriptional Factors, PDX-1, NeuroD1, and MafA, Causes Differentiation of Bone Marrow Mesenchymal Stem Cells into Insulin-Producing Cells. Experimental Diabetes Research. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-489250
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-489250
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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