miRNA589 Regulates Epithelial-Mesenchymal Transition in Human Peritoneal Mesothelial Cells
المؤلفون المشاركون
Peng, You-ming
Zhang, Ke
Zhou, Xun
Liu, Ying-hong
Xiao, Li
Tang, Wen-bin
Sun, Lin
Liu, Hong
Liu, Fu-you
Zhang, Hao
المصدر
Journal of Biomedicine and Biotechnology
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-10-03
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Background.
microRNA (miRNA, miR) are thought to interact with multiple mRNAs which are involved in the EMT process.
But the role of miRNAs in peritoneal fibrosis has remained unknown.
Objective.
To determine if miRNA589 regulates the EMT induced by TGFβ1 in human peritoneal mesothelial cell line (HMrSV5 cells).
Methods.
1.
Level of miR589 was detected in both human peritoneal mesothelial cells (HPMCs) isolated from continuous ambulatory peritoneal dialysis (CAPD) patients’ effluent and HMrSV5 cells treated with or without TGFβ1.
2.
HMrSV5 cells were divided into three groups: control group, TGFβ1 group, and pre-miR-589+TGFβ1 group.
The level of miRNA589 was determined by realtime PCR.
The expressions of ZO-1, vimentin, and E-cadherin in HPMCs were detected, respectively.
Results.
Decreased level of miRNA589 was obtained in either HPMCs of long-term CAPD patients or HMrSV5 cells treated with TGFβ1.
In vitro, TGFβ1 led to upregulation of vimentin and downregulation of ZO-1 as well as E-cadherin in HMrSV5 cells, which suggested EMT, was induced.
The changes were accompanied with notably decreased level of miRNA589 in HMrSV5 cells treated with TGFβ1.
Overexpression of miRNA589 by transfection with pre-miRNA589 partially reversed these EMT changes.
Conclusion.
miRNA589 mediates TGFβ1 induced EMT in human peritoneal mesothelial cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Ke& Zhang, Hao& Zhou, Xun& Tang, Wen-bin& Xiao, Li& Liu, Ying-hong…[et al.]. 2012. miRNA589 Regulates Epithelial-Mesenchymal Transition in Human Peritoneal Mesothelial Cells. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-489362
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Ke…[et al.]. miRNA589 Regulates Epithelial-Mesenchymal Transition in Human Peritoneal Mesothelial Cells. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-489362
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Ke& Zhang, Hao& Zhou, Xun& Tang, Wen-bin& Xiao, Li& Liu, Ying-hong…[et al.]. miRNA589 Regulates Epithelial-Mesenchymal Transition in Human Peritoneal Mesothelial Cells. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-489362
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-489362
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر