Towards Scarless Wound Healing : A Comparison of Protein Expression between Human, Adult and Foetal Fibroblasts
المؤلفون المشاركون
Foster, Leslie John Ray
Ho, Sonia
Marçal, Helder
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-01-30
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Proteins from human adult and foetal fibroblast cell lines were compared, focusing on those involved in wound healing.
Proteins were separated through two-dimensional gel electrophoresis (2DE).
Differences in protein spot intensity between the lineages were quantified through 3D gel scanning densitometry.
Selected protein spots were excised, subjected to tryptic digests, prior to separation using HPLC with a linear ion trap mass spectrometer, and identified.
Protein maps representing the proteomes from adult and foetal fibroblasts showed similar distributions but revealed differences in expression levels.
Heat shock cognate 71 kDA protein, Tubulin alpha-1A chain, actin cytoplasmic-1, and neuron cytoplasmic protein were all expressed in significantly higher concentrations by foetal fibroblasts, nearly double those observed for their adult counterparts.
Fructose bisphosphate aldolase A, Cofilin-1, Peroxiredoxin-1, Lactotransferrin Galectin-1, Profilin-1, and Calreticulin were expressed at comparatively higher concentrations by the adult fibroblasts.
Significant differences in the expression levels of some proteins in human adult and foetal fibroblasts correlated with known differences in wound healing behaviour.
This data may assist in the development of technologies to promote scarless wound healing and better functional tissue repair and regeneration.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ho, Sonia& Marçal, Helder& Foster, Leslie John Ray. 2014. Towards Scarless Wound Healing : A Comparison of Protein Expression between Human, Adult and Foetal Fibroblasts. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-489681
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ho, Sonia…[et al.]. Towards Scarless Wound Healing : A Comparison of Protein Expression between Human, Adult and Foetal Fibroblasts. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-489681
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ho, Sonia& Marçal, Helder& Foster, Leslie John Ray. Towards Scarless Wound Healing : A Comparison of Protein Expression between Human, Adult and Foetal Fibroblasts. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-489681
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-489681
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر