![](/images/graphics-bg.png)
Apoptosis Effect of Girinimbine Isolated from Murraya koenigii on Lung Cancer Cells In Vitro
المؤلفون المشاركون
Abdelwahab, Siddig Ibrahim
Shamsuddin, Noorasyikin
Syam, Suvitha
Mohan, Syam
Sukari, Mohd Aspollah
Cheah, Shiau-Chuen
Rais Mustafa, Mohd
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-03-13
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Murraya koenigii Spreng has been traditionally claimed as a remedy for cancer.
The current study investigated the anticancer effects of girinimbine, a carbazole alkaloid isolated from Murraya koenigii Spreng, on A549 lung cancer cells in relation to apoptotic mechanistic pathway.
Girinimbine was isolated from Murraya koenigii Spreng.
The antiproliferative activity was assayed using MTT and the apoptosis detection was done by annexin V and lysosomal stability assays.
Multiparameter cytotoxicity assays were performed to investigate the change in mitochondrial membrane potential and cytochrome c translocation.
ROS, caspase, and human apoptosis proteome profiler assays were done to investigate the apoptotic mechanism of cell death.
The MTT assay revealed that the girinimbine induces cell death with an IC50 of 19.01 μM.
A significant induction of early phase of apoptosis was shown by annexin V and lysosomal stability assays.
After 24 h treatment with 19.01 μM of girinimbine, decrease in the nuclear area and increase in mitochondrial membrane potential and plasma membrane permeability were readily visible.
Moreover the translocation of cytochrome c also was observed.
Girinimbine mediates its antiproliferative and apoptotic effects through up- and downregulation of apoptotic and antiapoptotic proteins.
There was a significant involvement of both intrinsic and extrinsic pathways.
Moreover, the upregulation of p53 as well as the cell proliferation repressor proteins, p27 and p21, and the significant role of insulin/IGF-1 signaling were also identified.
Moreover the caspases 3 and 8 were found to be significantly activated.
Our results taken together indicated that girinimbine may be a potential agent for anticancer drug development.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mohan, Syam& Abdelwahab, Siddig Ibrahim& Cheah, Shiau-Chuen& Sukari, Mohd Aspollah& Syam, Suvitha& Shamsuddin, Noorasyikin…[et al.]. 2013. Apoptosis Effect of Girinimbine Isolated from Murraya koenigii on Lung Cancer Cells In Vitro. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-490776
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mohan, Syam…[et al.]. Apoptosis Effect of Girinimbine Isolated from Murraya koenigii on Lung Cancer Cells In Vitro. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-490776
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mohan, Syam& Abdelwahab, Siddig Ibrahim& Cheah, Shiau-Chuen& Sukari, Mohd Aspollah& Syam, Suvitha& Shamsuddin, Noorasyikin…[et al.]. Apoptosis Effect of Girinimbine Isolated from Murraya koenigii on Lung Cancer Cells In Vitro. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-490776
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-490776
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)