Enzymatic Synthesis of the Flavone Glucosides, Prunin and Isoquercetin, and the Aglycones, Naringenin and Quercetin, with Selective α-L-Rhamnosidase and β-D-Glucosidase Activities of Naringinase
المؤلفون المشاركون
Vila-Real, Hélder
Bronze, M. Rosário
Alfaia, António J.
Calado, António R. T.
Ribeiro, Maria H. L.
المصدر
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-09-15
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
The production of flavonoid glycosides by removing rhamnose from rutinosides can be accomplished through enzymatic catalysis.
Naringinase is an enzyme complex, expressing both α-L-rhamnosidase and β-D-glucosidase activities, with application in glycosides hydrolysis.
To produce monoglycosylated flavonoids with naringinase, the expression of β-D-glucosidase activity is not desirable leading to the need of expensive methods for α-L-rhamnosidase purification.
Therefore, the main purpose of this study was the inactivation of β-D-glucosidase activity expressed by naringinase keeping α-L-rhamnosidase with a high retention activity.
Response surface methodology (RSM) was used to evaluate the effects of temperature and pH on β-D-glucosidase inactivation.
A selective inactivation of β-D-glucosidase activity of naringinase was achieved at 81.5∘C and pH 3.9, keeping a very high residual activity of α-L-rhamnosidase (78%).
This was a crucial achievement towards an easy and cheap production method of very expensive flavonoids, like prunin and isoquercetin starting from naringin and rutin, respectively.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Vila-Real, Hélder& Alfaia, António J.& Bronze, M. Rosário& Calado, António R. T.& Ribeiro, Maria H. L.. 2011. Enzymatic Synthesis of the Flavone Glucosides, Prunin and Isoquercetin, and the Aglycones, Naringenin and Quercetin, with Selective α-L-Rhamnosidase and β-D-Glucosidase Activities of Naringinase. Enzyme Research،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-491024
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Vila-Real, Hélder…[et al.]. Enzymatic Synthesis of the Flavone Glucosides, Prunin and Isoquercetin, and the Aglycones, Naringenin and Quercetin, with Selective α-L-Rhamnosidase and β-D-Glucosidase Activities of Naringinase. Enzyme Research No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-491024
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Vila-Real, Hélder& Alfaia, António J.& Bronze, M. Rosário& Calado, António R. T.& Ribeiro, Maria H. L.. Enzymatic Synthesis of the Flavone Glucosides, Prunin and Isoquercetin, and the Aglycones, Naringenin and Quercetin, with Selective α-L-Rhamnosidase and β-D-Glucosidase Activities of Naringinase. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-491024
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-491024
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر