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Cationic Polyene Phospholipids as DNA Carriers for Ocular Gene Therapy
المؤلفون المشاركون
Pungente, Michael D.
Oliveira, Ana Vanessa
Silva, Gabriela A.
Calado, Sofia
Machado, Susana
Øpstad, Christer L.
Bitoque, Diogo
Sliwka, Hans-Richard
Partali, Vassilia
Zeeshan, Muhammad
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-07-24
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Recent success in the treatment of congenital blindness demonstrates the potential of ocular gene therapy as a therapeutic approach.
The eye is a good target due to its small size, minimal diffusion of therapeutic agent to the systemic circulation, and low immune and inflammatory responses.
Currently, most approaches are based on viral vectors, but efforts continue towards the synthesis and evaluation of new nonviral carriers to improve nucleic acid delivery.
Our objective is to evaluate the efficiency of novel cationic retinoic and carotenoic glycol phospholipids, designated C20-18, C20-20, and C30-20, to deliver DNA to human retinal pigmented epithelium (RPE) cells.
Liposomes were produced by solvent evaporation of ethanolic mixtures of the polyene compounds and coformulated with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) or cholesterol (Chol).
Addition of DNA to the liposomes formed lipoplexes, which were characterized for binding, size, biocompatibility, and transgene efficiency.
Lipoplex formulations of suitable size and biocompatibility were assayed for DNA delivery, both qualitatively and quantitatively, using RPE cells and a GFP-encoding plasmid.
The retinoic lipoplex formulation with DOPE revealed a transfection efficiency comparable to the known lipid references 3β-[N-(N′,N′-dimethylaminoethane)-carbamoyl]-cholesterol (DC-Chol) and 1,2-dioleoyl-sn-glycero-3-ethylphosphocholine (EPC) and GeneJuice.
The results demonstrate that cationic polyene phospholipids have potential as DNA carriers for ocular gene therapy.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Machado, Susana& Calado, Sofia& Bitoque, Diogo& Oliveira, Ana Vanessa& Øpstad, Christer L.& Zeeshan, Muhammad…[et al.]. 2014. Cationic Polyene Phospholipids as DNA Carriers for Ocular Gene Therapy. BioMed Research International،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-491809
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Machado, Susana…[et al.]. Cationic Polyene Phospholipids as DNA Carriers for Ocular Gene Therapy. BioMed Research International No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-491809
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Machado, Susana& Calado, Sofia& Bitoque, Diogo& Oliveira, Ana Vanessa& Øpstad, Christer L.& Zeeshan, Muhammad…[et al.]. Cationic Polyene Phospholipids as DNA Carriers for Ocular Gene Therapy. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-491809
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-491809
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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