The Characterization of High-Fat Diet and Multiple Low-Dose Streptozotocin Induced Type 2 Diabetes Rat Model
المؤلفون المشاركون
Lv, Xiao-Yan
Xu, Zhi-Gang
Chen, Li
Li, Jing
Zhang, Ming
المصدر
Experimental Diabetes Research
العدد
المجلد 2008، العدد 2008 (31 ديسمبر/كانون الأول 2008)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2009-01-04
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Aim.
Based on the previously established method, we developed a better and stable animal model of type 2 diabetes mellitus by high-fat diet combined with multiple low-dose STZ injections.
Meanwhile, this new model was used to evaluate the antidiabetic effect of berberine.
Method.
Wistar male rats fed with regular chow for 4 weeks received vehicle (control groups), rats fed with high-fat diet for 4 weeks received different amounts of STZ once or twice by intraperitoneal injection (diabetic model groups), and diabetic rats were treated with berberine (100 mg/kg, berberine treatment group).
Intraperitoneal glucose tolerance test and insulin tolerance test were carried out.
Moreover, fasting blood glucose, fasting insulin, total cholesterol, and triglyceride were measured to evaluate the dynamic blood sugar and lipid metabolism.
Result.
The highest successful rate (100%) was observed in rats treated with a single injection of 45 mg/kg STZ, but the plasma insulin level of this particular group was significantly decreased, and ISI has no difference compared to control group.
The successful rate of 30 mg/kg STZ twice injection group was significantly high (85%) and the rats in this group presented a typical characteristic of T2DM as insulin resistance, hyperglycemia, and blood lipid disorder.
All these symptoms observed in the 30 mg/kg STZ twice injection group were recovered by the treatment of berberine.
Conclusion.
Together, these results indicated that high-fat diet combined with multiple low doses of STZ (30 mg/kg at weekly intervals for 2 weeks) proved to be a better way for developing a stable animal model of type 2 diabetes, and this new model may be suitable for pharmaceutical screening.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Ming& Lv, Xiao-Yan& Li, Jing& Xu, Zhi-Gang& Chen, Li. 2009. The Characterization of High-Fat Diet and Multiple Low-Dose Streptozotocin Induced Type 2 Diabetes Rat Model. Experimental Diabetes Research،Vol. 2008, no. 2008, pp.1-9.
https://search.emarefa.net/detail/BIM-491887
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Ming…[et al.]. The Characterization of High-Fat Diet and Multiple Low-Dose Streptozotocin Induced Type 2 Diabetes Rat Model. Experimental Diabetes Research No. 2008 (2008), pp.1-9.
https://search.emarefa.net/detail/BIM-491887
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Ming& Lv, Xiao-Yan& Li, Jing& Xu, Zhi-Gang& Chen, Li. The Characterization of High-Fat Diet and Multiple Low-Dose Streptozotocin Induced Type 2 Diabetes Rat Model. Experimental Diabetes Research. 2009. Vol. 2008, no. 2008, pp.1-9.
https://search.emarefa.net/detail/BIM-491887
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-491887
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر