Differential Expression and Immunolocalization of Antioxidant Enzymes in Entamoeba histolytica Isolates during Metronidazole Stress
المؤلفون المشاركون
Paul, Jaishree
Singh, Nishant
Iyer, Lakshmi Rani
Verma, Anil Kumar
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-06-12
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Entamoeba histolytica infections are endemic in the Indian subcontinent.
Five to eight percent of urban population residing under poor sanitary conditions suffers from Entamoeba infections.
Metronidazole is the most widely prescribed drug used for amoebiasis.
In order to understand the impact of metronidazole stress on the parasite, we evaluated the expression of two antioxidant enzymes, peroxiredoxin and FeSOD, in Entamoeba histolytica isolates during metronidazole stress.
The results reveal that, under metronidazole stress, the mRNA expression levels of these enzymes did not undergo any significant change.
Interestingly, immunolocalization studies with antibodies targeting peroxiredoxin indicate differential localization of the protein in the cell during metronidazole stress.
In normal conditions, all the Entamoeba isolates exhibit presence of peroxiredoxin in the nucleus as well as in the membrane; however with metronidazole stress the protein localized mostly to the membrane.
The change in the localization pattern was more pronounced when the cells were subjected to short term metronidazole stress compared to cells adapted to metronidazole.
The protein localization to the cell membrane could be the stress response mechanism in these isolates.
Colocalization pattern of peroxiredoxin with CaBp1, a cytosolic protein, revealed that the membrane and nuclear localization was specific to peroxiredoxin during metronidazole stress.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Iyer, Lakshmi Rani& Singh, Nishant& Verma, Anil Kumar& Paul, Jaishree. 2014. Differential Expression and Immunolocalization of Antioxidant Enzymes in Entamoeba histolytica Isolates during Metronidazole Stress. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-491960
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Iyer, Lakshmi Rani…[et al.]. Differential Expression and Immunolocalization of Antioxidant Enzymes in Entamoeba histolytica Isolates during Metronidazole Stress. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-491960
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Iyer, Lakshmi Rani& Singh, Nishant& Verma, Anil Kumar& Paul, Jaishree. Differential Expression and Immunolocalization of Antioxidant Enzymes in Entamoeba histolytica Isolates during Metronidazole Stress. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-491960
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-491960
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر