Superior Silencing by 2′,4′-BNANC-Based Short Antisense Oligonucleotides Compared to 2′,4′-BNALNA-Based Apolipoprotein B Antisense Inhibitors
المؤلفون المشاركون
Yasuhara, Hidenori
Harada-Shiba, Mariko
Obika, Satoshi
Yamamoto, Tsuyoshi
Imanishi, Takeshi
Wada, Fumito
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-09-26
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
The duplex stability with target mRNA and the gene silencing potential of a novel bridged nucleic acid analogue are described.
The analogue, 2′,4′-BNANC antisense oligonucleotides (AONs) ranging from 10- to 20-nt-long, targeted apolipoprotein B.
2′,4′-BNANC was directly compared to its conventional bridged (or locked) nucleic acid (2′,4′-BNA/LNA)-based counterparts.
Melting temperatures of duplexes formed between 2′,4′-BNANC-based antisense oligonucleotides and the target mRNA surpassed those of 2′,4′-BNA/LNA-based counterparts at all lengths.
An in vitro transfection study revealed that when compared to the identical length 2′,4′-BNA/LNA-based counterpart, the corresponding 2′,4′-BNANC-based antisense oligonucleotide showed significantly stronger inhibitory activity.
This inhibitory activity was more pronounced in shorter (13-, 14-, and 16-mer) oligonucleotides.
On the other hand, the 2′,4′-BNANC-based 20-mer AON exhibited the highest affinity but the worst IC50 value, indicating that very high affinity may undermine antisense potency.
These results suggest that the potency of AONs requires a balance between reward term and penalty term.
Balance of these two parameters would depend on affinity, length, and the specific chemistry of the AON, and fine-tuning of this balance could lead to improved potency.
We demonstrate that 2′,4′-BNANC may be a better alternative to conventional 2′,4′-BNA/LNA, even for “short” antisense oligonucleotides, which are attractive in terms of drug-likeness and cost-effective bulk production.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yamamoto, Tsuyoshi& Yasuhara, Hidenori& Wada, Fumito& Harada-Shiba, Mariko& Imanishi, Takeshi& Obika, Satoshi. 2012. Superior Silencing by 2′,4′-BNANC-Based Short Antisense Oligonucleotides Compared to 2′,4′-BNALNA-Based Apolipoprotein B Antisense Inhibitors. Journal of Nucleic Acids،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-492167
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yamamoto, Tsuyoshi…[et al.]. Superior Silencing by 2′,4′-BNANC-Based Short Antisense Oligonucleotides Compared to 2′,4′-BNALNA-Based Apolipoprotein B Antisense Inhibitors. Journal of Nucleic Acids No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-492167
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yamamoto, Tsuyoshi& Yasuhara, Hidenori& Wada, Fumito& Harada-Shiba, Mariko& Imanishi, Takeshi& Obika, Satoshi. Superior Silencing by 2′,4′-BNANC-Based Short Antisense Oligonucleotides Compared to 2′,4′-BNALNA-Based Apolipoprotein B Antisense Inhibitors. Journal of Nucleic Acids. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-492167
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-492167
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر