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Aberrant Methylation of Thrombospondin-1 and Its Association with Reduced Expression in Gastric Cardia Adenocarcinoma
المؤلفون المشاركون
Yang, Zhibin
Chen, Zhifeng
Guo, Wei
Kuang, Gang
Dong, Zhiming
He, Ming
Guo, Jianwen
Guo, Yanli
المصدر
Journal of Biomedicine and Biotechnology
العدد
المجلد 2010، العدد 2010 (31 ديسمبر/كانون الأول 2010)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2010-03-15
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Aim.
Investigate the promoter methylation of the Thrombospondin-1 (TSP1) gene in gastric cardia adenocarcinoma (GCA).
Methods.
MSP approach, immunohistochemistry method, and RT-PCR were used respectively to examine the promoter methylation of TSP1, its protein and mRNA expression in tumors and corresponding normal tissues.
The expression and concentration of TGF-β1 were examined respectively by immunohistochemistry and ELISA method.
The status of T cell immunity was examined by Flow cytometry analysis.
Results.
TSP1 was methylated in 34/96 (35.4%) tumor specimens, which was significantly higher than that in corresponding normal tissues (P<.001).
Protein and mRNA expression of TSP1 in GCA tumor tissues were reduced significantly and were associated with TSP1 methylation.
The protein expression of TGF-β1 was significantly higher in tumor tissues (P<.001) and was associated with TNM stage and histological differentiation.
The concentration of active and total TGF-β1 did not show significant difference between the GCA patients with hypermethylation of TSP1 and without methylation of TSP1 (P>.05).
The function of T cell immunity was significantly different between the GCA patients with hypermethylation of TSP1 and without methylation of TSP1.
Conclusions.
Epigenetic silencing of TSP1 gene by promoter hypermethylation may play an important role in GCA.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Guo, Wei& Dong, Zhiming& He, Ming& Guo, Yanli& Guo, Jianwen& Chen, Zhifeng…[et al.]. 2010. Aberrant Methylation of Thrombospondin-1 and Its Association with Reduced Expression in Gastric Cardia Adenocarcinoma. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-493341
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Guo, Wei…[et al.]. Aberrant Methylation of Thrombospondin-1 and Its Association with Reduced Expression in Gastric Cardia Adenocarcinoma. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-10.
https://search.emarefa.net/detail/BIM-493341
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Guo, Wei& Dong, Zhiming& He, Ming& Guo, Yanli& Guo, Jianwen& Chen, Zhifeng…[et al.]. Aberrant Methylation of Thrombospondin-1 and Its Association with Reduced Expression in Gastric Cardia Adenocarcinoma. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-493341
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-493341
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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